Wang Yanlin, Zhu Guijin, Li Xia, Li Xiaolan
Reproductive Medical Center, Department of Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2006;26(3):353-5. doi: 10.1007/BF02829574.
Taking the mouse as a model, the experimental method of observing the morphology of meiotic spindles and chromosomes in mature oocytes were investigated in order to evaluate the effects of various interventions on the quality of oocytes accurately and rapidly. Laser scanning confocal microscope (LSCM) was used to examine the meiotic spindles and chromosomes by the technologies of optical section and three-dimensional (3D) image reconstruction. The results showed that the configurations of meiotic spindles and chromosomes could be observed clearly by LSCM. The normal rate of meiotic spindles and chromosomes was 82% and 86% respectively. It was concluded that the LSCM was a valid instrument to observe the meiotic spindles and chromosomes of mature oocytes and could be used as a valid method to evaluate the quality of M II oocytes.
以小鼠为模型，研究了观察成熟卵母细胞减数分裂纺锤体和染色体形态的实验方法，以便准确、快速地评估各种干预措施对卵母细胞质量的影响。利用激光扫描共聚焦显微镜（LSCM）通过光学切片和三维（3D）图像重建技术检测减数分裂纺锤体和染色体。结果表明，通过LSCM可以清晰观察到减数分裂纺锤体和染色体的形态。减数分裂纺锤体和染色体的正常率分别为82%和86%。结论是，LSCM是观察成熟卵母细胞减数分裂纺锤体和染色体的有效仪器，可作为评估MⅡ期卵母细胞质量的有效方法。
Zotero 插件