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lpr/lpr小鼠中CD4+细胞亚群：T细胞受体/CD3复合物表达及增殖反应的差异

Subpopulations of CD4+ cells in lpr/lpr mice: differences in expression of T cell receptor/CD3 complex and proliferative responses.

作者信息

Asano T, Yoshikai Y, Matsumoto K, Matuzaki G, Nomoto K

机构信息

Department of Immunology, Kyushu University, Fukuoka, Japan.

出版信息

Clin Exp Immunol. 1990 Jul;81(1):90-6. doi: 10.1111/j.1365-2249.1990.tb05296.x.

DOI:10.1111/j.1365-2249.1990.tb05296.x
PMID:1696186
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1535029/
Abstract

CD4+ cells from autoimmune-prone C57BL/6 lpr/lpr mice contain two subpopulations, B220-CD4+ and B220+CD4+ cells. Highly purified B220-CD4+ cells from C57BL/6 +/+ and lpr/lpr mice were examined by comparing functional characteristics and expression of cell surface antigens and T cell receptor (TcR)/CD3 complex. Both lpr B220+CD4+ and B220+CD4-CD8- cells, most of which were PgP-1 positive, expressed TcR/CD3 complex on the cell surface at lower level as compared with B220-CD4+ cells of age-matched normal mice. In addition, the B2200-CD4+ cells were heterogeneous on the basis of surface expression of PgP-1 and CD3 antigens. Normal levels of TcR C alpha-, C beta- and V beta 8-specific mRNA were found in the B220-CD4+ cells and B220+CD4+ cells as compared with normal B220-CD4+ cells, while V beta 8-specific mRNA was preferentially expressed only by B220+CD4-CD8- cells. Either B220+CD4+ cells and B220+CD4-CD8- cells failed to respond to anti-CD3 monoclonal antibody (MoAb) as assessed by proliferative responses and production of interleukin-2 (IL-2). However, appreciable levels of reactivity to anti-CD3 MoAb were detected in the B220-CD4+ cells, although the responsiveness of this subset to such stimuli were reduced, compared with those of normal control. These results indicate that the B220-CD4+ cells in lpr mice are phenotypically and functionally distinct from normal B220-CD4+ cells.

摘要

自身免疫易感的C57BL/6 lpr/lpr小鼠的CD4+细胞包含两个亚群，即B220-CD4+细胞和B220+CD4+细胞。通过比较功能特性、细胞表面抗原表达以及T细胞受体（TcR）/CD3复合物，对来自C57BL/6 +/+和lpr/lpr小鼠的高度纯化的B220-CD4+细胞进行了检测。与年龄匹配的正常小鼠的B220-CD4+细胞相比，lpr B220+CD4+细胞和B220+CD4-CD8-细胞（其中大多数为PgP-1阳性）在细胞表面表达TcR/CD3复合物的水平较低。此外，基于PgP-1和CD3抗原的表面表达，B2200-CD4+细胞具有异质性。与正常B220-CD4+细胞相比，在B220-CD4+细胞和B220+CD4+细胞中发现了正常水平的TcR Cα-、Cβ-和Vβ8特异性mRNA，而Vβ8特异性mRNA仅在B220+CD4-CD8-细胞中优先表达。通过增殖反应和白细胞介素-2（IL-2）的产生评估，B220+CD4+细胞和B220+CD4-CD8-细胞均未对抗CD3单克隆抗体（MoAb）产生反应。然而，在B220-CD4+细胞中检测到了对抗CD3 MoAb的明显反应水平，尽管该亚群对这种刺激的反应性与正常对照相比有所降低。这些结果表明，lpr小鼠中的B220-CD4+细胞在表型和功能上与正常B220-CD4+细胞不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bb8/1535029/006e4eb9ad62/clinexpimmunol00070-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bb8/1535029/006e4eb9ad62/clinexpimmunol00070-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6bb8/1535029/006e4eb9ad62/clinexpimmunol00070-0095-a.jpg

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本文引用的文献

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Flow cytometry analysis of T cells and continuous T-cell lines from autoimmune MRL/l mice.对来自自身免疫性MRL/l小鼠的T细胞和连续T细胞系进行流式细胞术分析。
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The role of T3 in the activation of human T cells.T3在人T细胞活化中的作用。
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9
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J Immunol. 1984 Jun;132(6):2686-9.
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Inositol trisphosphate, a novel second messenger in cellular signal transduction.肌醇三磷酸，细胞信号转导中的一种新型第二信使。
Nature. 1984;312(5992):315-21. doi: 10.1038/312315a0.