针对人T细胞克隆受体的单克隆抗体的抗原样效应。
Antigen-like effects of monoclonal antibodies directed at receptors on human T cell clones.
作者信息
Meuer S C, Hodgdon J C, Hussey R E, Protentis J P, Schlossman S F, Reinherz E L
出版信息
J Exp Med. 1983 Sep 1;158(3):988-93. doi: 10.1084/jem.158.3.988.
Abstract
Recent studies suggested that the clonally unique Ti epitopes defined by non-cross-reactive monoclonal antibodies might represent the variable regions of the antigen receptor. Here we determine whether such anti-Ti antibodies could trigger clonal T cell activation. Anticlonotypic monoclonal antibodies to the 49/43-kdalton heterodimer of a given clone or antibodies to the 20/25-kdalton membrane associated monomorphic T3 molecule selectively induce proliferation and IL-2 secretion when linked to a solid support. In contrast, anti-T4 and anti-T8 antibodies under the same conditions have no effect. In conclusion, these results imply that anticlonotypic antibody functions in a fashion analogous to antigen and further support the notion that the T3-Ti molecular complex represents the antigen receptor on human T lymphocytes.
摘要
最近的研究表明，由非交叉反应性单克隆抗体所界定的克隆独特性Ti表位可能代表抗原受体的可变区。在此我们确定此类抗Ti抗体是否能够触发克隆性T细胞激活。针对给定克隆的49/43-kdalton异二聚体的抗克隆型单克隆抗体或针对20/25-kdalton膜相关单态性T3分子的抗体，当与固相载体连接时可选择性地诱导增殖和白细胞介素-2分泌。相比之下，相同条件下的抗T4和抗T8抗体则无作用。总之，这些结果表明抗克隆型抗体的作用方式类似于抗原，并进一步支持了T3-Ti分子复合物代表人类T淋巴细胞上的抗原受体这一观点。
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本文引用的文献
1
Antigen recognition by human T lymphocytes is linked to surface expression of the T3 molecular complex.人类T淋巴细胞对抗原的识别与T3分子复合物的表面表达相关联。
Cell. 1982 Oct;30(3):735-43. doi: 10.1016/0092-8674(82)90278-1.
2
Surface structures involved in target recognition by human cytotoxic T lymphocytes.参与人类细胞毒性T淋巴细胞识别靶标的表面结构。
Science. 1982 Oct 29;218(4571):471-3. doi: 10.1126/science.6981845.
3
Clonal analysis of human cytotoxic T lymphocytes: T4+ and T8+ effector T cells recognize products of different major histocompatibility complex regions.人细胞毒性T淋巴细胞的克隆分析：T4 +和T8 +效应T细胞识别不同主要组织相容性复合体区域的产物。
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4395-9. doi: 10.1073/pnas.79.14.4395.
4
5
OKT3: a monoclonal anti-human T lymphocyte antibody with potent mitogenic properties.OKT3：一种具有强大促有丝分裂特性的单克隆抗人T淋巴细胞抗体。
J Immunol. 1980 Jun;124(6):2708-13.
6
Comparison of T3-associated 49- and 43-kilodalton cell surface molecules on individual human T-cell clones: evidence for peptide variability in T-cell receptor structures.个体人类T细胞克隆上与T3相关的49千道尔顿和43千道尔顿细胞表面分子的比较：T细胞受体结构中肽变异性的证据。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4104-8. doi: 10.1073/pnas.80.13.4104.
7
Antibody directed at a surface structure inhibits cytolytic but not suppressor function of human T lymphocytes.针对表面结构的抗体可抑制人T淋巴细胞的细胞溶解功能，但不抑制其抑制功能。
Nature. 1981 Nov 12;294(5837):168-70. doi: 10.1038/294168a0.
8
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Eur J Immunol. 1980 Oct;10(10):758-62. doi: 10.1002/eji.1830101006.
9
10
Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor.作为T细胞抗原受体的与T3相关的90K异二聚体的证据。
Nature. 1983 Jun 30;303(5920):808-10. doi: 10.1038/303808a0.
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