Chong Kong T, Xiang Liangbin, Wang Xiaohong, Jun Eunjoo L, Xi Long-Fu, Schweinfurth John M
Department of Otolaryngology & Communicative Sciences, University of Mississippi Medical Center, Mississippi, USA.
Virol J. 2006 Sep 8;3:75. doi: 10.1186/1743-422X-3-75.
Epithelial defensins including human beta-defensins (hBDs) and alpha-defensins (HDs) are antimicrobial peptides that play important roles in the mucosal defense system. However, the role of defensins in papillomavirus induced epithelial lesions is unknown.
Papilloma tissues were prospectively collected from 15 patients with recurrent respiratory papillomatosis (RRP) and analyzed for defensins and chemokine IL-8 expression by quantitative, reverse-transcriptase polymerase chain reaction (RT-PCR) assays. HBD-1, -2 and -3 mRNAs were detectable in papilloma samples from all RRP patients and the levels were higher than in normal oral mucosal tissues from healthy individuals. Immunohistochemical analysis showed that both hBD-1 and 2 were localized in the upper epithelial layers of papilloma tissues. Expression of hBD-2 and hBD-3 appeared to be correlated as indicated by scatter plot analysis (r = 0.837, p < 0.01) suggesting that they were co-inducible in papillomavirus induced lesions. Unlike hBDs, only low levels of HD5 and HD6 were detectable in papillomas and in oral mucosa.
Human beta-defensins are upregulated in respiratory papillomas. This novel finding suggests that hBDs might contribute to innate and adaptive immune responses targeted against papillomavirus-induced epithelial lesions.
包括人β-防御素（hBDs）和α-防御素（HDs）在内的上皮防御素是抗菌肽，在黏膜防御系统中发挥重要作用。然而，防御素在乳头瘤病毒诱导的上皮病变中的作用尚不清楚。
前瞻性收集了15例复发性呼吸道乳头瘤病（RRP）患者的乳头瘤组织，通过定量逆转录聚合酶链反应（RT-PCR）分析防御素和趋化因子白细胞介素-8（IL-8）的表达。在所有RRP患者的乳头瘤样本中均可检测到HBD-1、-2和-3的mRNA，其水平高于健康个体的正常口腔黏膜组织。免疫组织化学分析显示，hBD-1和-2均定位于乳头瘤组织的上皮上层。散点图分析表明，hBD-2和hBD-3的表达似乎具有相关性（r = 0.837，p < 0.01），提示它们在乳头瘤病毒诱导的病变中可共同诱导表达。与hBDs不同，在乳头瘤和口腔黏膜中仅可检测到低水平的HD5和HD6。
人β-防御素在呼吸道乳头瘤中上调。这一新颖发现提示hBDs可能有助于针对乳头瘤病毒诱导的上皮病变的固有免疫和适应性免疫反应。
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