Bissell John, Joly Sophie, Johnson Georgia K, Organ Connie C, Dawson Deborah, McCray Paul B, Guthmiller Janet M
Dows Institute for Dental Research, Department of Periodontics, College of Dentistry, University of Iowa, IA 52242, USA.
J Oral Pathol Med. 2004 May;33(5):278-85. doi: 10.1111/j.0904-2512.2004.00143.x.
Human beta-defensins (HBDs) are epithelial-derived antimicrobial peptides. The expression of three HBDs (HBD-1, HBD-2, and HBD-3) has been reported in oral mucosa, gingiva, and salivary glands. However, their role in protection against oral infections is not well understood. This study examined the expression of HBD-1, HBD-2, and HBD-3 mRNAs in gingival health and periodontal disease.
Gingival tissue discarded from periodontal procedures was obtained from 20 periodontally healthy and 29 periodontally diseased sites. Total RNA was isolated, and expression of beta-defensins was analyzed by both 35-cycle and semiquantitative (25-cycle) reverse transcription-polymerase chain reaction (RT-PCR). The level of expression was assigned ordinal scores of no expression, low expression, or high expression. mRNA expression was compared between healthy and diseased groups using exact tests of homogeneity and Cochran-Mantel-Haenszel tests; associations among the variables were assessed using exact tests and Kendall's tau-b statistic.
All 49 samples demonstrated basal mRNA expression of HBD-1, HBD-2, and HBD-3. Significantly higher levels of HBD-3 (P = 0.012) expression were found in the healthy tissues as compared to the diseased ones. There was also a suggestion of higher expression of HBD-2 in the healthy tissues (P = 0.12). Levels of HBD-1, HBD-2, and HBD-3 mRNA expression were correlated with one another (P < 0.001).
High levels of HBD-3 mRNA expression in healthy tissues suggest a potentially important protective role for defensins in the host immune response to infection by periodontal pathogens.
人β-防御素（HBDs）是上皮来源的抗菌肽。已报道在口腔黏膜、牙龈和唾液腺中有三种HBDs（HBD-1、HBD-2和HBD-3）表达。然而，它们在预防口腔感染中的作用尚未完全明确。本研究检测了HBD-1、HBD-2和HBD-3 mRNA在牙龈健康和牙周疾病中的表达情况。
从20个牙周健康部位和29个牙周疾病部位获取牙周手术中丢弃的牙龈组织。提取总RNA，通过35个循环和半定量（25个循环）逆转录聚合酶链反应（RT-PCR）分析β-防御素的表达。将表达水平分为无表达、低表达或高表达的序贯评分。使用精确同质性检验和 Cochr an-Mantel-Haenszel检验比较健康组和疾病组之间的mRNA表达；使用精确检验和Kendall's tau-b统计量评估变量之间的关联。
所有49个样本均显示HBD-1、HBD-2和HBD-3的基础mRNA表达。与患病组织相比，健康组织中HBD-3的表达水平显著更高（P = 0.012）。健康组织中HBD-2的表达也有升高的趋势（P = 0.12）。HBD-1、HBD-2和HBD-3 mRNA表达水平相互关联（P < 0.001）。
健康组织中高水平的HBD-3 mRNA表达表明防御素在宿主对牙周病原体感染的免疫反应中可能具有重要的保护作用。
