Labruna Marcelo B, McBride Jere W, Camargo Luis Marcelo A, Aguiar Daniel M, Yabsley Michael J, Davidson William R, Stromdahl Ellen Y, Williamson Phillip C, Stich Roger W, Long S Wesley, Camargo Erney P, Walker David H
Department of Pathology, University of Texas Medical Branch, Galveston, TX 7755-0609, USA.
Vet Parasitol. 2007 Jan 31;143(2):189-95. doi: 10.1016/j.vetpar.2006.08.005. Epub 2006 Sep 7.
A molecular epidemiologic investigation in two Brazilian states (Rondônia and São Paulo) was undertaken to determine if Ehrlichia species responsible for human and animal ehrlichioses in North America could be found in Brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. Samples, including 376 ticks comprising 9 Amblyomma species, 29 capybara (Hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile patients with history of tick bites were tested by a real-time PCR assay targeting a fragment of the Ehrlichia dsb gene. Ehrlichia DNA was not detected in any tick, capybara or human samples. In contrast, 4 out of 5 dogs contained Ehrlichia canis DNA in their blood, which were sequenced, representing the first report of E. canis infecting dogs in the Amazon region of Brazil. Further studies are needed to evaluate the presence of other agents of human and animal ehrlichioses in Brazil.
在巴西的两个州（朗多尼亚州和圣保罗州）开展了一项分子流行病学调查，以确定在巴西的媒介、潜在的天然哺乳动物宿主以及有蜱叮咬史的发热人类患者中，是否能发现导致北美人类和动物埃立克体病的埃立克体物种。通过针对埃立克体dsb基因片段的实时PCR检测法，对包括376只蜱（分属9种钝缘蜱）、29只水豚（Hydrochaeris hydrochaeris）脾脏、5份犬类血液以及75份有蜱叮咬史的发热患者的人类血液样本在内的样本进行了检测。在任何蜱、水豚或人类样本中均未检测到埃立克体DNA。相比之下，5只犬中有4只血液中含有犬埃立克体DNA，并对其进行了测序，这是巴西亚马逊地区犬埃立克体感染犬类的首次报告。需要进一步研究以评估巴西是否存在其他导致人类和动物埃立克体病的病原体。
