Fernandez N, Puente P, Leal F
Departamento de Microbiología, Genética, Medicina Preventiva, Universidad de Salamanca, Spain.
FEMS Microbiol Lett. 1990 May;57(1-2):7-11. doi: 10.1016/0378-1097(90)90404-e.
A FITC-dextran internalization assay with Saccharomyces cerevisiae as positive control was used to determine whether fluid-phase endocytosis is a general characteristic of yeasts. Schizosaccharomyces pombe, Pichia polymorpha, Kluyveromyces phaseolosporus, Yarrowia lipolytica and Candida albicans were clearly positive, whereas results obtained with Debaryomyces marama were inconclusive. In all cases internalized FITC-dextran was found to be localized in the vacuoles and the process was always time- and temperature-dependent. Lower eucaryotes, particularly yeasts, appear to have the ability to incorporate substances from the extracellular medium through fluid-phase endocytosis.
以酿酒酵母作为阳性对照，进行了异硫氰酸荧光素标记葡聚糖内化试验，以确定液相内吞作用是否为酵母的普遍特征。粟酒裂殖酵母、多形毕赤酵母、菜豆克鲁维酵母、解脂耶氏酵母和白色念珠菌的试验结果均为明显阳性，而马腊德巴利酵母的试验结果尚无定论。在所有情况下，均发现内化的异硫氰酸荧光素标记葡聚糖定位于液泡中，且该过程始终依赖时间和温度。低等真核生物，尤其是酵母，似乎具有通过液相内吞作用从细胞外介质中摄取物质的能力。
