膳食鱼油可减少猪心室肌细胞早期后去极化的发生。
Dietary fish oil reduces the occurrence of early afterdepolarizations in pig ventricular myocytes.
作者信息
Den Ruijter Hester M, Verkerk Arie O, Berecki Géza, Bakker Diane, van Ginneken Antoni C G, Coronel Ruben
机构信息
Department of Clinical and Experimental Cardiology, Experimental and Molecular Cardiology Groups, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
出版信息
J Mol Cell Cardiol. 2006 Nov;41(5):914-7. doi: 10.1016/j.yjmcc.2006.08.001. Epub 2006 Sep 11.
Fish oil reduces sudden cardiac death in post myocardial infarction patients. Life-threatening arrhythmias in heart failure are associated with repolarization abnormalities leading to EAD(1) formation. We examined the effects of incorporated fish oil omega3-PUFAs(2) on EAD formation in pig myocytes. Pigs were fed a diet rich in fish oil or sunflower oil (control) for 8 weeks. Myocytes were isolated by enzymatic dissociation and patch-clamped. Susceptibility to EAD formation was tested using E4031 (5 microM), a blocker of I(Kr). The fish oil diet in pigs resulted in increased incorporation of omega3-PUFAs in the sarcolemma of the myocytes compared to the control diet and caused a reduced occurrence of E4031-induced EADs in pig myocytes. A shorter action potential, a reduced action potential prolongation in response to E-4031 and a reduced reactivation of I(Ca,L) by omega3-PUFAs may explain the observed reduction in EADs. A diet rich in fish oil protects against EAD formation.
鱼油可降低心肌梗死后患者的心源性猝死。心力衰竭中危及生命的心律失常与复极异常有关，而复极异常会导致早后去极化（EAD）（1）的形成。我们研究了掺入的鱼油ω-3多不饱和脂肪酸（2）对猪心肌细胞中EAD形成的影响。给猪喂食富含鱼油或向日葵油（对照）的饮食8周。通过酶解分离心肌细胞并进行膜片钳记录。使用I（Kr）阻滞剂E4031（5微摩尔）测试EAD形成的易感性。与对照饮食相比，猪的鱼油饮食导致ω-3多不饱和脂肪酸在心肌细胞膜中的掺入增加，并使猪心肌细胞中E4031诱导的EADs发生率降低。较短的动作电位、对E-4031反应时动作电位延长的减少以及ω-3多不饱和脂肪酸对L型钙电流（I（Ca,L））再激活的减少可能解释了观察到的EADs减少现象。富含鱼油的饮食可预防EAD的形成。
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