Grainger David C, Hurd Douglas, Goldberg Martin D, Busby Stephen J W
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Nucleic Acids Res. 2006;34(16):4642-52. doi: 10.1093/nar/gkl542. Epub 2006 Sep 8.
The Escherichia coli chromosome is condensed into an ill-defined structure known as the nucleoid. Nucleoid-associated DNA-binding proteins are involved in maintaining this structure and in mediating chromosome compaction. We have exploited chromatin immunoprecipitation and high-density microarrays to study the binding of three such proteins, FIS, H-NS and IHF, across the E.coli genome in vivo. Our results show that the distribution of these proteins is biased to intergenic parts of the genome, and that the binding profiles overlap. Hence some targets are associated with combinations of bound FIS, H-NS and IHF. In addition, many regions associated with FIS and H-NS are also associated with RNA polymerase.
大肠杆菌染色体浓缩形成一种定义不明确的结构，称为类核。类核相关的DNA结合蛋白参与维持这种结构并介导染色体压缩。我们利用染色质免疫沉淀和高密度微阵列技术，在体内研究了三种此类蛋白（FIS、H-NS和IHF）在大肠杆菌基因组中的结合情况。我们的结果表明，这些蛋白的分布偏向于基因组的基因间区域，并且结合图谱相互重叠。因此，一些靶点与结合的FIS、H-NS和IHF的组合相关。此外，许多与FIS和H-NS相关的区域也与RNA聚合酶相关。
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