Chao D, Chen Y A, Liu J K, Huang T C
Department of Biology, National Sun Yat-Sen University, Kaohsiung, Taiwan, Republic of China.
Proc Natl Sci Counc Repub China B. 1990 Jan;14(1):54-8.
Crithidia fasciculata, Leishmania donovani, Leishmania major, Leishmania mexicana amazonensis, Leishmania tropica, Leishmania tarentolae, Trypanosoma sp. from Formosan bats (Tb), Trypanosoma lewisi, Trypanosoma musculi, and different strains of Trypanosoma cruzi (Tc) were cultivated at 27 degrees C in a liquid culture medium. Flagellates harvested from log phase culture were analyzed for their lectin agglutinating characteristics with concanavalin A (Con A), Peanut agglutinin, Ricinus communis agglutinin 120, soybean agglutinin (SBA), Ulex europeus agglutinin (UEA) and wheat germ agglutinin (WGA). Results indicated that all these flagellates might have D-galactose and methyl- alpha-D-manopyranoside on their surface. The presence of L-Fucose, which complexes specifically with UEA, could not be demonstrated on the surface of these flagellates. Results from quantitative comparison of surface molecules of Tb and the Tulahuen strain of Tc suggested that Tb may have more WGA-binding molecules while Tc may have more ConA-binding molecules. Pretreatment of the flagellates with 0.05% trypsin at 37 degrees C for 30 minutes caused some reduction of agglutination titers. Cell agglutination with lectins was completely inhibited or reversed in the presence of the specific lectin-binding monosaccharides.
将克氏锥虫、杜氏利什曼原虫、硕大利什曼原虫、亚马逊利什曼原虫、热带利什曼原虫、塔氏利什曼原虫、来自台湾蝙蝠的锥虫（Tb）、鲁氏锥虫、鼠锥虫以及不同株的克氏锥虫（Tc）在27摄氏度的液体培养基中培养。对对数期培养收获的鞭毛虫用伴刀豆球蛋白A（Con A）、花生凝集素、蓖麻凝集素120、大豆凝集素（SBA）、荆豆凝集素（UEA）和小麦胚芽凝集素（WGA）分析其凝集素凝集特性。结果表明，所有这些鞭毛虫表面可能都有D-半乳糖和α-D-吡喃甘露糖苷。在这些鞭毛虫表面未证实有与UEA特异性结合的L-岩藻糖存在。对Tb和Tc的图拉温株表面分子进行定量比较的结果表明，Tb可能有更多与WGA结合的分子，而Tc可能有更多与ConA结合的分子。在37摄氏度用0.05%胰蛋白酶对鞭毛虫预处理30分钟会使凝集效价有所降低。在存在特异性凝集素结合单糖的情况下，凝集素引起的细胞凝集被完全抑制或逆转。
