用于研究博尔纳病病毒的反向遗传学方法。

Reverse-genetic approaches to the study of Borna disease virus.

作者信息

de la Torre Juan C

机构信息

Molecular Integrative Neuroscience Department IMM-6, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

出版信息

Nat Rev Microbiol. 2006 Oct;4(10):777-83. doi: 10.1038/nrmicro1489. Epub 2006 Sep 11.

DOI:10.1038/nrmicro1489

PMID:16964271

Abstract

Borna disease virus (BDV) is an enveloped virus that has a non-segmented, negative-strand RNA genome with the characteristic organization of the mononegaviruses. However, based on its unique genetic and biological features, BDV is considered to be the prototypic member of a new mononegavirus family, the Bornaviridae. BDV causes central nervous system (CNS) disease in a wide variety of mammals. This article discusses the recently developed reverse-genetics systems for BDV, and the implications for the elucidation of the molecular mechanisms underlying BDV-host interactions, including the basis of BDV persistence in the CNS and its associated diseases.

摘要

博尔纳病病毒（BDV）是一种包膜病毒，具有不分节段的负链RNA基因组，其基因组结构具有单股负链RNA病毒的典型特征。然而，基于其独特的遗传和生物学特性，BDV被认为是新的单股负链RNA病毒科——博尔纳病毒科的原型成员。BDV可在多种哺乳动物中引发中枢神经系统（CNS）疾病。本文讨论了最近开发的BDV反向遗传学系统，以及该系统对于阐明BDV与宿主相互作用的分子机制的意义，包括BDV在中枢神经系统中持续存在及其相关疾病的基础。