Amit-Romach Einat, Reifen Ram, Uni Zehava
Department of Animal Science, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Int J Mol Med. 2006 Oct;18(4):721-7.
Many studies dealing with trinitrobenzene sulfonic acid (TNBS) colitis in rats have been carried out referring only to the colon. In humans, ulcerative colitis (UC) can extend a variable distance into the terminal ileum in a phenomenon known as backwash ileitis (BWI). The aim of this study was therefore to examine the effect of TNBS-induced colitis on different aspects of the rat ileum and jejunum. We hypothesized that TNBS administration would lead to a systemic influence on the small intestine. Rats were induced colitis by administration of 0.25 ml of 2,4,6-trinitrobenzene sulfonic acid and 72 h after colitis induction animals were sacrificed. Segments were taken of the colon, ileum and jejunum. In addition to mucin mRNA expression, morphological changes were observed in the jejunum and ileum. We examined the mRNA expression and biochemical activity of brush border enzyme, sucrase iso-maltase and aminopeptidase, in all three segments. The villous surface area of colitis-induced rats was smaller in jejunum and ileum compared to control. In the jejunum of TNBS-induced rats, goblet-cell volume increased and their density decreased. The relative amount of MUC2 mRNA decreased in the jejunum, ileum and colon of colitis rat. However, MUC3 mRNA expression increased in the ileum and colon of these rats. Sucrase isomaltase expression and activity decreased in the ileum of TNBS-induced rats, while aminopeptidase activity was lower in the jejunum. These observations suggest that intrarectal administration of TNBS to rats influences not only their colon and terminal ileum, but also the proximal ileum and jejunum. Involvement of the ileum and jejunum in TNBS-induced colitis may be related to the systemic reaction of the immune system and mucosa to colitis.
许多关于大鼠三硝基苯磺酸（TNBS）结肠炎的研究仅涉及结肠。在人类中，溃疡性结肠炎（UC）可延伸至末端回肠一段可变的距离，即所谓的反流性回肠炎（BWI）。因此，本研究的目的是考察TNBS诱导的结肠炎对大鼠回肠和空肠不同方面的影响。我们假设给予TNBS会对小肠产生全身性影响。通过给予0.25 ml 2,4,6 - 三硝基苯磺酸诱导大鼠结肠炎，在诱导结肠炎72小时后处死动物。取结肠、回肠和空肠的组织段。除了检测黏蛋白mRNA表达外，还观察了空肠和回肠的形态变化。我们检测了所有三个组织段刷状缘酶、蔗糖酶异麦芽糖酶和氨肽酶的mRNA表达及生化活性。与对照组相比，结肠炎诱导大鼠的空肠和回肠绒毛表面积较小。在TNBS诱导大鼠的空肠中杯状细胞体积增加而密度降低。结肠炎大鼠空肠、回肠和结肠中MUC2 mRNA的相对含量降低。然而，这些大鼠回肠和结肠中MUC3 mRNA表达增加。TNBS诱导大鼠回肠中蔗糖酶异麦芽糖酶表达及活性降低，而空肠中氨肽酶活性较低。这些观察结果表明，向大鼠直肠内给予TNBS不仅影响其结肠和末端回肠，还影响近端回肠和空肠。回肠和空肠参与TNBS诱导的结肠炎可能与免疫系统和黏膜对结肠炎的全身性反应有关。
