Jurjus Abdo, Barada Kassem, Khoury Naim, Assef Mona Diab, Foltzer Charlotte Jourdainne, Reimund Jean Marie, Kedinger Michele
Faculty of Medicine, American University of Beirut, Riad El Solh, Beirut 1107-2020, Lebanon.
Can J Physiol Pharmacol. 2006 Nov;84(11):1191-203. doi: 10.1139/y06-069.
This study aims to describe the morphological alterations in the small and large intestines as well as the expression of some enterocyte enzymes and carriers in a rat model of iodoacetamide-induced colitis. Biopsies from the large and small intestines were taken at 1, 2, 4, 8, and 16 days postinduction and studied by light microscopy. The expressions of lactase, sucrase, aminopeptidase, and Glut-5 in the jejunum were studied by immunohistochemistry. Gene expressions of enterocyte lactase and sucrase were determined by RT-PCR using specific oligonucleotides. Microscopic examination of the large intestines revealed manifestations concordant with inflammation. Such alterations peaked at 2 days, were maintained to a lesser extent for 4 days, regressed by 8 days, and healed by 16 days. In the jejunum, the expression of lactase, sucrase, and aminopeptidase decreased 2 days after colitis induction, and recovered 2 days later. Similarly, Glut-5 expression decreased transiently with partial recovery by day 8. Compared with sham, gene expression of jejunal brush border enzymes sucrase and lactase showed a 4-fold increase in lactase and a 9-fold increase in sucrase after 4 days. We conclude that colitis can induce significant functional abnormalities in distant noninflamed small bowel regions.
本研究旨在描述碘乙酰胺诱导的大鼠结肠炎模型中小肠和大肠的形态学改变，以及一些肠上皮细胞酶和载体的表达情况。在诱导后1、2、4、8和16天采集大肠和小肠活检组织，通过光学显微镜进行研究。采用免疫组织化学方法研究空肠中乳糖酶、蔗糖酶、氨肽酶和Glut-5的表达。使用特异性寡核苷酸通过RT-PCR测定肠上皮细胞乳糖酶和蔗糖酶的基因表达。大肠的显微镜检查显示出与炎症相符的表现。这些改变在2天时达到峰值，在4天时程度较轻地持续存在，在8天时消退，并在16天时愈合。在空肠中，结肠炎诱导后2天乳糖酶、蔗糖酶和氨肽酶的表达下降，2天后恢复。同样，Glut-5表达短暂下降，到第8天部分恢复。与假手术组相比，空肠刷状缘酶蔗糖酶和乳糖酶的基因表达在4天后乳糖酶增加4倍，蔗糖酶增加9倍。我们得出结论，结肠炎可在远处未发炎的小肠区域诱导显著的功能异常。
