Kudryashova I V, Kudryashov I E, Gulyaeva N V
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 5a Butlerov Street, 117485 Moscow, Russia.
Neurosci Behav Physiol. 2006 Oct;36(8):817-24. doi: 10.1007/s11055-006-0092-y.
Treatment of hippocampal slices with the caspase-3 inhibitor Z-DEVD-FMK led to a decrease in the magnitude of long-term potentiation (LTP), which developed over time. Testing with paired stimuli separated by an interval of 70 msec showed that after caspase-3 inhibition, as compared with control slices, the second response in the pair showed no increase in amplitude in conditions of LTP. In these conditions, the magnitude of LTP depended on differences in the amplitudes of the first and second responses before induction of LTP. LTP was absent in slices with initially highly efficient afferent stimulation and correspondingly low levels of facilitation in paired-pulse stimulation. It is suggested that inhibition of caspase-3 prevents the structural rearrangements in LTP associated with the involvement of new synapses and neurons in the response.
用半胱天冬酶-3抑制剂Z-DEVD-FMK处理海马切片会导致随着时间推移而形成的长时程增强（LTP）幅度降低。用间隔70毫秒的成对刺激进行测试表明，与对照切片相比，在半胱天冬酶-3受到抑制后，在LTP条件下成对刺激中的第二个反应幅度没有增加。在这些条件下，LTP的幅度取决于LTP诱导前第一个和第二个反应幅度的差异。在最初传入刺激高效且相应地成对脉冲刺激中易化水平较低的切片中不存在LTP。有人提出，抑制半胱天冬酶-3可防止LTP中与新突触和神经元参与反应相关的结构重排。
