Imam A, Stathopoulos E, Taylor C R
Department of Pathology, University of Southern California, School of Medicine, Los Angeles 90033.
Anticancer Res. 1990 Jul-Aug;10(4):1095-104.
Anti-BLA.36 is an antibody that recognizes a glycoprotein with an apparent molecular weight of 36 kilodaltons, termed B lymphocyte antigen (BLA.36). By using an immunochemical staining technique, BLA.36 was found to be specifically expressed on Hodgkin's and human B cell lines including early B progenitor cells. Other cell lines representing T cell lymphomas, non-B large cell lymphomas, melanomas and carcinomas were consistently negative. BLA.36 is distinct from the previously identified antigens of hematopoietic cell lineage. The specificity of expression of BLA.36 in tissue sections mirrored that of cell lines. In normal tissues, BLA.36 was detectable predominantly on cells in the germinal center and mantle zone of reactive follicles in lymph nodes and spleens. In hematopoietic malignancy, the antigen was expressed on the surface of Reed-Sternberg cells, mononuclear Hodgkin's cells and also on malignant cells of B cell lineage. BLA.36 was also observed on lymphoid cells of 10 to 24 week fetal liver: a double-antibody-staining method revealed that these BLA.36-positive cells also contained immunoglobulin mu heavy chain consistent with identification as early B cells. Under these conditions, T lymphocytes, histiocytes, granulocytes, macrophages, stromal cells in lymphoid tissue, and both normal and neoplastic epithelial cells were consistently negative for the expression of the antigen, with the single exception of a variable proportion of Kupffer cells in normal liver. The antibody has already established its usefulness for the identification of Reed-Sternberg and Hodgkin's cells, and also normal and malignant B lymphocytes in frozen as well as formalin-fixed tissue sections. Furthermore, binding of F(ab)2 fragments of anti-BLA.36 to antigen-positive cell lines specifically inhibited the proliferation of cells. Such an effect was eliminated by the removal of the antibody from the culture-medium, suggesting a possible growth-related function of the antigen in Hodgkin's and B cells.
抗BLA.36是一种能识别一种表观分子量为36千道尔顿的糖蛋白的抗体，该糖蛋白被称为B淋巴细胞抗原（BLA.36）。通过使用免疫化学染色技术，发现BLA.36在霍奇金淋巴瘤细胞和包括早期B祖细胞在内的人B细胞系中特异性表达。代表T细胞淋巴瘤、非B大细胞淋巴瘤、黑色素瘤和癌的其他细胞系始终呈阴性。BLA.36与先前鉴定的造血细胞谱系抗原不同。BLA.36在组织切片中的表达特异性与细胞系中的情况相似。在正常组织中，BLA.36主要在淋巴结和脾脏反应性滤泡的生发中心和套区的细胞上可检测到。在造血系统恶性肿瘤中，该抗原在里德-斯腾伯格细胞、单核霍奇金细胞以及B细胞系的恶性细胞表面表达。在10至24周龄胎儿肝脏的淋巴细胞上也观察到了BLA.36：一种双抗体染色方法显示，这些BLA.36阳性细胞还含有免疫球蛋白μ重链，这与鉴定为早期B细胞一致。在这些条件下，T淋巴细胞、组织细胞、粒细胞、巨噬细胞、淋巴组织中的基质细胞以及正常和肿瘤上皮细胞对抗原表达始终呈阴性，正常肝脏中可变比例的库普弗细胞是唯一的例外。该抗体已被证明可用于在冰冻以及福尔马林固定的组织切片中鉴定里德-斯腾伯格细胞和霍奇金细胞，以及正常和恶性B淋巴细胞。此外，抗BLA.36的F(ab)2片段与抗原阳性细胞系的结合特异性抑制了细胞的增殖。通过从培养基中去除抗体，这种作用消失了，这表明该抗原在霍奇金细胞和B细胞中可能具有与生长相关的功能。
