Nikandrov V N, Zhuk O N
Laboratory for Regulatory Proteins and Peptides, Institute of Physiology, Belarus National Academy of Sciences, Minsk.
Neurosci Behav Physiol. 2006 Oct;36(8):841-5. doi: 10.1007/s11055-006-0095-8.
The aim of the present work was to study the effects of streptokinase (SK) on the ultrastructure of the cellular elements of the cerebral cortex of neonatal rats in vitro. Three series of cultures were used: cultures maintained in DMEM enriched with 15% fetal calf serum (control 1), some transferred to minimal nutritive medium containing only 0.5% serum (control 2), and some supplemented with SK (2000 MU/ml) (experimental). Addition of SK to the nutritive medium prevented a decrease in the viability of cells in a mature (14 days) dissociated culture from the neocortex of rat pups aged 1-2 days induced by transfer of the cultures to medium lacking serum proteins. The action of SK on 7-day cultures enhanced the loss of cell viability. Electron microscopy studies of organotypic cultures showed that at this concentration, SK prevented the development of destructive changes of astrocytes, oligodendrocytes, and neurons induced in explants by serum protein deficiency. Neurons showed an abundance of mitochondria, some of which had few cristae. Signs of destruction affected only the nuclei of neurons. After exposure to SK for 48 h, oligodendrocytes were activated (they contained an abundance of myelin-like bodies), with degradation of most astrocytes (surviving examples showing nuclear hyperchromicity). Neurons were resistant to these effects.
本研究的目的是在体外研究链激酶（SK）对新生大鼠大脑皮质细胞成分超微结构的影响。使用了三组培养物：在富含15%胎牛血清的DMEM中培养的（对照1），一些转移到仅含0.5%血清的基本营养培养基中的（对照2），以及一些添加了SK（2000 MU/ml）的（实验）。向营养培养基中添加SK可防止将培养物转移至缺乏血清蛋白的培养基所诱导的1 - 2日龄大鼠幼崽新皮质成熟（14天）解离培养物中细胞活力的降低。SK对7天培养物的作用增强了细胞活力的丧失。器官型培养物的电子显微镜研究表明，在此浓度下，SK可防止血清蛋白缺乏在外植体中诱导的星形胶质细胞、少突胶质细胞和神经元的破坏性变化。神经元有大量线粒体，其中一些线粒体嵴较少。破坏迹象仅影响神经元的细胞核。暴露于SK 48小时后，少突胶质细胞被激活（它们含有大量髓鞘样小体），大多数星形胶质细胞发生降解（存活的例子显示核染色质增多）。神经元对这些影响具有抗性。
