King A, Loke Y W
Cambridge University Department of Pathology, United Kingdom.
Cell Immunol. 1990 Sep;129(2):435-48. doi: 10.1016/0008-8749(90)90219-h.
Freshly isolated decidual large granular lymphocytes (LGL) show natural killer (NK) activity against K562 cells but not against normal human trophoblast. We now show that these decidual LGL proliferate in vitro in response to recombinant interleukin-2 (rIL-2) and that these rIL-2-stimulated cells acquire a broad cytolytic potential that is characteristic of lymphokine-activated killer (LAK) cells. Both fetal fibroblasts and JEG-3 choriocarcinoma cells are resistant to lysis by freshly isolated decidual effectors but are readily killed by IL-2-stimulated decidual LGL. The ability to kill these target cells is acquired after only 18 hr exposure to rIL-2. rIL-2-activated decidual LGL also kill cultured normal trophoblast cells but much lower levels of cytolysis were seen even after the effectors had been stimulated with rIL-2 for 4-6 days. The preferential killing of malignant over normal human trophoblast cells raises questions about the potential role of IL-2-activated decidual LGL in the control of unduly invasive or malignant trophoblast populations in vivo.
新鲜分离的蜕膜大颗粒淋巴细胞（LGL）对K562细胞显示出自然杀伤（NK）活性，但对正常人滋养层细胞无此活性。我们现在发现，这些蜕膜LGL在体外对重组白细胞介素-2（rIL-2）有增殖反应，并且这些经rIL-2刺激的细胞获得了具有淋巴因子激活的杀伤（LAK）细胞特征的广泛细胞溶解潜能。胎儿成纤维细胞和JEG-3绒毛膜癌细胞对新鲜分离的蜕膜效应细胞的裂解有抗性，但很容易被IL-2刺激的蜕膜LGL杀死。仅在暴露于rIL-2 18小时后就获得了杀死这些靶细胞的能力。rIL-2激活的蜕膜LGL也能杀死培养的正常滋养层细胞，但即使效应细胞用rIL-2刺激4 - 6天后，细胞溶解水平也低得多。与正常人类滋养层细胞相比，对恶性滋养层细胞的优先杀伤引发了关于IL-2激活的蜕膜LGL在体内控制过度侵袭性或恶性滋养层细胞群体中潜在作用的问题。
