Ferry B L, Sargent I L, Starkey P M, Redman C W
Harris Birthright Centre for Perinatal Medicine, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Cell Immunol. 1991 Jan;132(1):140-9. doi: 10.1016/0008-8749(91)90013-2.
Large granular lymphocytes (LGL) are the most abundant cell type in first trimester human pregnancy decidua. We have shown previously that CD56-positive decidual LGL have cytotoxic activity against the natural killer (NK) target K562, and that this cytotoxicity is augmented by pretreatment with interleukin-2 (IL-2). We now report that flow cytometrically purified populations of CD56-positive decidual LGL have no cytotoxic activity against either the BeWo choriocarcinoma cell line or freshly isolated term trophoblast. Incubation of unfractionated decidual cells with IL-2 induced cytotoxicity against BeWo, but term trophoblast remained resistant to lysis. Both BeWo and trophoblast showed much lower binding frequencies to decidual or peripheral blood cells than K56 targets, and excess trophoblast did not inhibit cytotoxic activity against K562. This suggests that the resistance of trophoblast to lysis by either decidual or peripheral blood LGL is due to the lack of accessible NK target structures on the surface of trophoblast.
大颗粒淋巴细胞（LGL）是孕早期人妊娠蜕膜中最丰富的细胞类型。我们之前已经表明，CD56阳性的蜕膜LGL对自然杀伤（NK）靶细胞K562具有细胞毒性活性，并且这种细胞毒性可通过白细胞介素-2（IL-2）预处理而增强。我们现在报告，通过流式细胞术纯化的CD56阳性蜕膜LGL群体对BeWo绒毛膜癌细胞系或新鲜分离的足月滋养层细胞均无细胞毒性活性。用IL-2孵育未分级的蜕膜细胞可诱导对BeWo的细胞毒性，但足月滋养层细胞仍对裂解具有抗性。BeWo和滋养层细胞与蜕膜或外周血细胞的结合频率均远低于K56靶细胞，并且过量的滋养层细胞不会抑制对K562的细胞毒性活性。这表明滋养层细胞对蜕膜或外周血LGL裂解的抗性是由于滋养层细胞表面缺乏可及的NK靶结构。
