Shaw Maren, Warren Simon, Groben Pamela, Gulley Margaret L
Department of Dermatology, University of North Carolina, Chapel Hill School of Medicine, Chapel Hill, NC 27599-7525, USA.
J Cutan Pathol. 2006 Sep;33(9):624-8. doi: 10.1111/j.1600-0560.2006.00498.x.
Merkel cell carcinoma (MCC) is an aggressive tumor of cutaneous neuroendocrine cells with a reported 13-fold increased incidence in immunocompromised patients, raising the possibility that it is driven by an oncogenic virus. Additionally, Merkel cell hyperplasia is seen in the Epstein-Barr virus (EBV)-driven process oral hairy leukoplakia, and EBV is known to be involved in the pathogenesis of several other malignancies.
We tested the hypothesis that EBV is involved in MCC.
We employed EBV-encoded RNA in situ hybridization (ISH), lytic EBV ISH, latent membrane protein 1 immunohistochemistry, and BamH1Z leftward reading frame 1 immunohistochemistry to detect and localize EBV in paraffin sections of MCC from five patients as well as seven other cutaneous tumors and positive controls for EBV infection.
Positive controls reacted appropriately. However, there was no evidence of latent or lytic EBV in any of the MCC biopsies or other cutaneous tumors.
Our findings suggest that EBV is not associated with MCC.
默克尔细胞癌（MCC）是一种皮肤神经内分泌细胞的侵袭性肿瘤，据报道免疫功能低下患者的发病率增加了13倍，这增加了其由致癌病毒驱动的可能性。此外，在爱泼斯坦-巴尔病毒（EBV）驱动的口腔毛状白斑过程中可见默克尔细胞增生，并且已知EBV参与其他几种恶性肿瘤的发病机制。
我们检验了EBV参与MCC发病的假说。
我们采用EBV编码RNA原位杂交（ISH）、EBV裂解期ISH、潜伏膜蛋白1免疫组织化学以及BamH1Z左向读框1免疫组织化学，来检测和定位5例患者的MCC石蜡切片以及其他7种皮肤肿瘤中的EBV，并设置EBV感染的阳性对照。
阳性对照反应正常。然而，在任何MCC活检组织或其他皮肤肿瘤中均未发现潜伏或裂解的EBV证据。
我们的研究结果表明EBV与MCC无关。
