Zhao C J, Wang C F, Deng X M, Gao Y, Wu Ch
Key Laboratories of Animal Breeding and Genetics of Ministry of Agriculture of P.R. China.
J Anim Breed Genet. 2006 Oct;123(5):349-52. doi: 10.1111/j.1439-0388.2006.00607.x.
5'-AMP-activated protein kinase plays an important role in regulating the level of ATP in the presence of metabolic stress. Previous studies revealed that polymorphisms in 5'-AMP-activated protein kinase gamma 3 subunit (PRKAG3) gene are associated with meat quality in pigs. In the present study, single-nucleotide polymorphisms (SNPs) in the 5'-end and exons of chicken PRKAG3 gene were identified with the method of single-strand conformation polymorphism in Hubbard ISA White broiler, Leghorn layer, and three Chinese indigenous chicken breeds, Tibet Chicken, Shouguang Chicken and Beijing Yellow Chicken. Two SNPs in the 5'-end of the gene and 10 SNPs in exons 3, 4, 9 and 11, of which three caused amino acid substitutions, were identified in the PRKAG3 gene of the five chicken breeds. The results will facilitate further study on the association between the mutations of PRKAG3 and chicken meat quality.
5'-AMP 激活蛋白激酶在代谢应激情况下调节三磷酸腺苷（ATP）水平方面发挥着重要作用。先前的研究表明，5'-AMP 激活蛋白激酶γ3 亚基（PRKAG3）基因的多态性与猪的肉质有关。在本研究中，采用单链构象多态性方法，在哈伯德艾维茵白肉鸡、来航蛋鸡以及三个中国地方鸡种藏鸡、寿光鸡和北京油鸡中，对鸡 PRKAG3 基因 5' 端和外显子的单核苷酸多态性（SNP）进行了鉴定。在这五个鸡种的 PRKAG3 基因中，鉴定出该基因 5' 端的两个 SNP 以及外显子 3、4、9 和 11 中的 10 个 SNP，其中三个导致了氨基酸替换。这些结果将有助于进一步研究 PRKAG3 突变与鸡肉品质之间的关联。
