过敏组胺分泌期间及之后大鼠肥大细胞三磷酸腺苷利用情况的评估
Estimation of adenosine triphosphate utilization of rat mast cells during and after anaphylactic histamine secretion.
作者信息
Johansen T
机构信息
Department of Pharmacology, Medical School, Odense University, Denmark.
出版信息
FEBS Lett. 1990 Jul 30;268(1):103-6. doi: 10.1016/0014-5793(90)80983-p.
Determination of the cellular content of adenosine triphosphate (ATP) and the rate of ATP-synthesis were used to estimate the cellular utilization of ATP in relation to anaphylactic histamine secretion. There was an increased rate of oxidative ATP-synthesis and a decreased cellular ATP content during the time period of histamine secretion and immediately after its completion. During secretion the additional ATP-utilization above the basal level of ATP-synthesis was 0.51 pmol/10(3) cells. 2.5 min after cell activation, the rate of additional ATP-utilization was 0.30 pmol/10(3) cells/min, and the persistent ATP-decrease observed after 30 and 120 min may be due to a decreased rate of oxidative ATP-synthesis.
通过测定三磷酸腺苷（ATP）的细胞含量和ATP合成速率，来评估细胞对ATP的利用与过敏性组胺分泌之间的关系。在组胺分泌期间及其刚结束后，氧化型ATP合成速率增加，而细胞内ATP含量降低。在分泌过程中，高于基础ATP合成水平的额外ATP利用率为0.51 pmol/10³个细胞。细胞激活后2.5分钟，额外ATP利用率为0.30 pmol/10³个细胞/分钟，30分钟和120分钟后观察到的持续性ATP减少可能是由于氧化型ATP合成速率降低所致。
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