在大鼠肥大细胞对化合物48/80作出反应分泌组胺期间及之后三磷酸腺苷的利用情况

Utilization of adenosine triphosphate in rat mast cells during and after secretion of histamine in response to compound 48/80.

作者信息

Johansen T

出版信息

Acta Pharmacol Toxicol (Copenh). 1983 Sep;53(3):245-9. doi: 10.1111/j.1600-0773.1983.tb01132.x.

DOI:10.1111/j.1600-0773.1983.tb01132.x

PMID:6195889

Abstract

The adenosine triphosphate (ATP) content of rat mast cells and their lactate production were measured during and after secretion of histamine induced by compound 48/80. Antimycin A and oligomycin were used to block oxidative ATP synthesis, and 2-deoxyglucose (2-DG) was used to block glycolytic ATP synthesis. Histamine secretion was completed after 10 sec. exposure of the cells to compound 48/80. During that time period there was an increased ATP-utilization of 0.15 pmol/10(3) cells. After completion of the secretory process there seemed to be an enhanced utilization of ATP of 0.40 pmol/10(3) cells/min., which may be associated with recovery of the cells.

摘要

在化合物48/80诱导大鼠肥大细胞分泌组胺的过程中及之后，对其三磷酸腺苷（ATP）含量和乳酸生成进行了测定。使用抗霉素A和寡霉素阻断氧化型ATP合成，使用2-脱氧葡萄糖（2-DG）阻断糖酵解型ATP合成。细胞暴露于化合物48/80 10秒后组胺分泌完成。在此期间，ATP利用率增加了0.15 pmol/10³个细胞。分泌过程完成后，似乎ATP利用率提高到了0.40 pmol/10³个细胞/分钟，这可能与细胞的恢复有关。