Gorczynski R M
Department of Surgery, University of Toronto, Ontario, Canada.
Immunol Lett. 1990 Jun;24(3):155-8. doi: 10.1016/0165-2478(90)90041-n.
Intravenous immunization of BALB/c mice with irradiated Leishmania mexicana slows the growth of a subsequent intradermal inoculation of virulent parasites. Prior subcutaneous immunization with irradiated parasites before i.v. immunization blocks the protective effect of the latter. Parasites harvested from vaccinated mice grow more slowly in naive mice than the initial inoculated clone, and have a diminished capacity to immunize mice against this initial clone when used as (irradiated) i.v. immunogen. However, parasites harvested from vaccinated mice are as effective as the initial clone in blocking protection when used as subcutaneous immunogen. Understanding the nature of this differential response in expression of protecting/suppressor determinants in parasites harvested from vaccinated or naive mice will likely be important to developing a suitable vaccination strategy for human use.
用经辐照的墨西哥利什曼原虫对BALB/c小鼠进行静脉免疫，可减缓随后皮内接种的强毒寄生虫的生长。在静脉免疫前先用经辐照的寄生虫进行皮下免疫，会阻断后者的保护作用。从接种疫苗的小鼠体内收获的寄生虫，在未接种的小鼠体内生长速度比最初接种的克隆体更慢，并且当用作（经辐照的）静脉免疫原时，其免疫小鼠抵抗该初始克隆体的能力减弱。然而，从接种疫苗的小鼠体内收获的寄生虫用作皮下免疫原时，在阻断保护方面与初始克隆体一样有效。了解从接种疫苗或未接种的小鼠体内收获的寄生虫中保护/抑制决定簇表达的这种差异反应的本质，可能对开发适用于人类的疫苗接种策略很重要。
