Gorczynski R M
Department of Surgery, Mount Sinai Research Institute, Toronto, Ontario, Canada.
Infect Immun. 1989 Aug;57(8):2430-3. doi: 10.1128/iai.57.8.2430-2433.1989.
BALB/c mice, which are normally highly susceptible to growth of Leishmania mexicana parasites in vivo, can be vaccinated with avirulent temperature-sensitive mutants of L. mexicana so that challenge with virulent organisms results in markedly diminished growth of the latter. Parasites extracted from the lesions which do appear in these mice are able to produce active infection in secondary hosts, although the rate of progression of these lesions is slower than that seen with the original virulent cloned organism. Interestingly, when irradiated parasites from the secondary hosts are themselves used to vaccinate naive BALB/c mice, less protection is seen than when irradiated virulent organisms from the initial infecting clone are used. These data suggest that when infection does take place in mice vaccinated with avirulent clones of parasite, the organisms which develop in lesions in these animals are substantially modified from those present in the initial infecting inoculum.
BALB/c小鼠通常在体内对墨西哥利什曼原虫寄生虫的生长高度敏感，可用无毒的温度敏感型墨西哥利什曼原虫突变体进行疫苗接种，从而在用毒性生物体进行攻击时，后者的生长会显著减少。从这些小鼠身上确实出现的病变中提取的寄生虫能够在二级宿主中产生活性感染，尽管这些病变的进展速度比最初的毒性克隆生物体慢。有趣的是，当用来自二级宿主的经辐射的寄生虫本身对未感染的BALB/c小鼠进行疫苗接种时，与使用来自初始感染克隆的经辐射的毒性生物体相比，所观察到的保护作用较小。这些数据表明，在用寄生虫无毒克隆进行疫苗接种的小鼠中发生感染时，在这些动物病变中形成的生物体与初始感染接种物中的生物体有很大不同。
