Yokoyama M M, Fujimoto K
Department of Immunology, Kurume University School of Medicine, Fukuoka, Japan.
Int J Tissue React. 1990;12(1):1-9.
Peripheral mononuclear cells taken from rheumatoid arthritis (RA) patients, which had previously been treated in vitro with the neuropeptide Substance P (SP) followed by stimulation with the mitogen PHA, showed a significantly higher percent positivity of lymphocytes with the double-markers CD4+ CD2+, CD8+ CD25+, CD4+, HLA-DR+ and CD8+ HLA-DR+ than those of the cells untreated by SP. No effect of SP was detected in normal controls, except that the cells with the double-marker CD4+ CD25+ were slightly increased. Furthermore, in RA patients the percentage of CD4+ CD45R- cells was increased, while that of CD4+ CD45R+ cells was decreased, by pre-treatment with SP and stimulation with PHA. Monocytes of patients with rheumatoid arthritis after the treatment with SP released a significantly higher amount of oxygen-intermediate than those of normal controls with or without SP. From these results, it could be considered that SP plays an important role in RA inflammation.
从类风湿性关节炎（RA）患者中获取的外周血单个核细胞，此前在体外先用神经肽P物质（SP）处理，然后用丝裂原PHA刺激，结果显示，与未经SP处理的细胞相比，具有双标记CD4 + CD2 +、CD8 + CD25 +、CD4 +、HLA - DR +和CD8 + HLA - DR +的淋巴细胞阳性百分比显著更高。在正常对照中未检测到SP的作用，只是具有双标记CD4 + CD25 +的细胞略有增加。此外，在RA患者中，通过SP预处理和PHA刺激，CD4 + CD45R -细胞的百分比增加，而CD4 + CD45R +细胞的百分比降低。用SP治疗后的类风湿性关节炎患者的单核细胞释放的氧中间体比有或没有SP的正常对照者的单核细胞释放的量显著更高。从这些结果可以认为，SP在RA炎症中起重要作用。
