Hanly J G, Pledger D, Parkhill W, Roberts M, Gross M
Department of Medicine, Victoria General Hospital, Halifax, NS, Canada.
J Rheumatol. 1990 Oct;17(10):1274-9.
The phenotypic characteristics of enzymatically dissociated synovial membrane mononuclear cells from 8 patients (14 samples) with rheumatoid arthritis (RA) were assessed by fluorescence activated flow cytometry and compared to peripheral blood (PB) mononuclear cells from 18 patients with RA and 14 normal controls. There was no significant difference between the percentage of CD4+ and CD8+ lymphocytes in synovial membrane compared to RA and normal PB. Double labelling experiments revealed similar percentages of CD4+ CDw29+ (helper-inducer) and CD4+ CD45R+ (suppressor-inducer) cells in RA and normal PB. In contrast, SM CD4+ CDw29+ cells were present in significantly higher proportions than in RA and normal PB (p less than 0.001). Conversely, synovial membrane CD4+ CD45R+ cells were present in significantly lower proportions than in RA and normal PB (p less than 0.001). A similar pattern of CDw29 and CD45R antigen expression was noted on CD8+ lymphocytes reflecting increased killer-effector (p less than 0.001) and decreased suppressor-effector (p less than 0.001) cells, respectively. Other experiments revealed a significant increase in the percentage of synovial membrane CD20+ cells (B lymphocytes) and HLA-DR+ cells compared to RA PB (p less than 0.02 and p less than 0.001) and normal PB (p less than 0.01 and p less than 0.005), and similar proportions of CD14+ cells (monocytes/macrophages). Our results suggest that RA synovial membrane contains populations of T and B lymphocytes that differ quantitatively and qualitatively from those in PB. These may account for some of the abnormalities in intraarticular humoral and cellular immune responses in patients with RA.
采用荧光激活流式细胞术对8例类风湿关节炎（RA）患者（14份样本）经酶解分离的滑膜膜单核细胞的表型特征进行评估，并与18例RA患者和14例正常对照者的外周血（PB）单核细胞进行比较。与RA患者和正常PB相比，滑膜膜中CD4 +和CD8 +淋巴细胞的百分比无显著差异。双标记实验显示，RA患者和正常PB中CD4 + CDw29 +（辅助诱导）和CD4 + CD45R +（抑制诱导）细胞的百分比相似。相比之下，滑膜膜CD4 + CDw29 +细胞的比例显著高于RA患者和正常PB（p小于0.001）。相反，滑膜膜CD4 + CD45R +细胞的比例显著低于RA患者和正常PB（p小于0.001）。在CD8 +淋巴细胞上观察到类似的CDw29和CD45R抗原表达模式，分别反映杀伤效应细胞增加（p小于0.001）和抑制效应细胞减少（p小于0.001）。其他实验显示，与RA患者PB（p小于0.02和p小于0.001）和正常PB（p小于0.01和p小于0.005）相比，滑膜膜CD20 +细胞（B淋巴细胞）和HLA - DR +细胞的百分比显著增加，CD14 +细胞（单核细胞/巨噬细胞）的比例相似。我们的结果表明，RA滑膜膜含有与PB中数量和质量不同的T和B淋巴细胞群体。这些可能是RA患者关节内体液和细胞免疫反应异常的部分原因。
