Chen Xiang-Li, Zhang Wang-Gang, Chen Xiao-Yan, Sun Zhong-Min, Liu Su-Hu
Department of Hematology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, P. R. China.
Ai Zheng. 2006 Sep;25(9):1134-7.
BACKGROUND & OBJECTIVE: Metastasis-associated protein S100A4 is overexpressed in many malignant tumor cells; it may play a pivotal role in invasion and metastasis of malignant tumors. This study was to determine the expression of S100A4 in human non-small cell lung cancer (NSCLC), and to investigate its correlations to invasion and metastasis of NSCLC.
The expression of S100A4 in 41 specimens of NSCLC and 6 specimens of normal lung tissues was detected by SP immunohistochemistry. The correlations of S100A4 to clinicopathologic features of NSCLC were analyzed.
The positive rate of S100A4 was significantly higher in NSCLC than in normal lung tissues (70.7% vs. 16.7%, P<0.05), and was significantly higher in adenocarcinoma than in squamous cell carcinoma (90.0% vs. 52.4%, P<0.01). The positive rate of S100A4 was significantly higher in stage III-IV than in stage II and stage I NSCLC (100.0% vs. 66.7% and 30.0%, P<0.01), while there was no obvious difference between the latter 2 groups (P>0.05). The positive rate of S100A4 was significantly higher in NSCLC with lymphatic metastasis than in NSCLC without lymphatic metastasis (90.0% vs. 52.4%, P<0.01), and significantly higher in NSCLC with tumor size of > or = 3 cm than in NSCLC with tumor size of < 3 cm (91.3% vs. 44.4%, P<0.001). The expression of S100A4 was closely related to lymphatic metastasis (r=0.480, P=0.001), and tumor size (r=0.288, P=0.017). No significant correlation was found between the expression of S100A4 and pathologic grade of NSCLC (P>0.05).
S100A4 expression is up-regulated in NSCLC, and closely related to lymphatic metastasis, TNM stage and tumor size, which suggest an important role of S100A4 in the invasion and metastasis of NSCLC.
转移相关蛋白S100A4在多种恶性肿瘤细胞中高表达，可能在恶性肿瘤的侵袭和转移中起关键作用。本研究旨在检测S100A4在人非小细胞肺癌（NSCLC）中的表达，并探讨其与NSCLC侵袭和转移的相关性。
采用SP免疫组化法检测41例NSCLC标本和6例正常肺组织中S100A4的表达，并分析S100A4与NSCLC临床病理特征的相关性。
NSCLC中S100A4的阳性率显著高于正常肺组织（70.7%对16.7%，P<0.05），腺癌中的阳性率显著高于鳞癌（90.0%对52.4%，P<0.01）。NSCLC Ⅲ-Ⅳ期S100A4的阳性率显著高于Ⅰ-Ⅱ期（100.0%对66.7%和30.0%，P<0.01），而Ⅰ-Ⅱ期之间无明显差异（P>0.05）。有淋巴结转移的NSCLC中S100A4的阳性率显著高于无淋巴结转移者（90.0%对52.4%，P<0.01），肿瘤大小≥3 cm的NSCLC中S100A4的阳性率显著高于肿瘤大小<3 cm者（91.3%对44.4%，P<0.001）。S100A4的表达与淋巴结转移（r=0.480，P=0.001）及肿瘤大小（r=0.288，P=0.017）密切相关。未发现S100A4的表达与NSCLC病理分级有显著相关性（P>0.05）。
NSCLC中S100A4表达上调，且与淋巴结转移、TNM分期及肿瘤大小密切相关，提示S100A4在NSCLC侵袭和转移中起重要作用。
