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雄性狨猴(普通狨猴和黑羽狨猴)肾脏中酶活性的组织化学定位。

Histochemical localization of enzyme activity in the kidneys of male marmosets (Callithrix jacchus and Callithrix penicillata).

作者信息

Miraglia T, Chavarria Irusta V R, Castro Filho B G, Pinto G

出版信息

Acta Anat (Basel). 1975;92(3):385-410.

PMID:169663
Abstract

The distribution of several hydrolases and oxidoreductases was studied in the renal parenchyma of adult male marmosets (Callithrix jacchus and Callithrix penicillata). The oxidative enzymes showed a high reactivity in the proximal and distal tubules, whereas the hydrolases reacted strongly in the proximal tubules but only weakly or not at all in the thick limb of Henle's loop, distal tubules and collecting ducts. The NAD-dependent enzymes (except alpha-GPDH) showed a stronger reactivity in the proximal tubules, while the NADP-dependent ones were more reactive in the thick limb of Henle's loop and distal convoluted tubules. Two groups of interstitial cells were found in the medulla. A first group inside the outer medulla, showing cells rich in acid phosphatase and nonspecific esterases and a second group, close to the papilla, reactive to a certain number of oxidative enzymes. A different reactivity in cells of the distal convoluted tubules, thick limb of Henle's loops and collecting ducts (dark cells) was seen in the case of some enzymes like nonspecific esterase, alpha-GPDH and SDH.

摘要

研究了几种水解酶和氧化还原酶在成年雄性狨猴(绢毛猴和黑羽狨)肾实质中的分布。氧化酶在近端和远端小管中显示出高反应性,而水解酶在近端小管中反应强烈,但在亨氏袢升支粗段、远端小管和集合管中反应微弱或无反应。NAD依赖的酶(α-GPDH除外)在近端小管中显示出更强的反应性,而NADP依赖的酶在亨氏袢升支粗段和远端曲管中反应性更强。在髓质中发现了两组间质细胞。第一组在外髓质内,显示富含酸性磷酸酶和非特异性酯酶的细胞,第二组靠近乳头,对一定数量的氧化酶有反应。在一些酶如非特异性酯酶、α-GPDH和SDH的情况下,在远端曲管、亨氏袢升支粗段和集合管(暗细胞)的细胞中观察到不同的反应性。

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Acta Anat (Basel). 1975;92(3):385-410.
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