Miraglia T, da Costa Nery J W, Costa Guedes M L
Acta Anat (Basel). 1976;96(1):97-114. doi: 10.1159/000144664.
The structure and histochemistry of the palmar and plantar skin were studied in four adult male marmosets (two Callithrix jacchus and two Callithrix penicillata). In this skin there exist well-developed epidermal ridges, to which are attached one or two ducts of sweat glands. A thick stratum corneum can be seen in the epidermis, while a distinct stratum lucidum cannot be isolated from the other layers. The stratum granulosum is constituted by one or three layers of cells containing keratohyalin granules. Melanin granulations are mainly concentrated in the basal cells of the epidermal ridges. Dendritic melanocytes and amelanotic melanocytes containing alkaline phosphatase are found among the epidermal cells. Glycogen, UDPG-GT and phosphorylases are mainly present in the middle and lower Malpighian cells of the epidermal ridges. Alkaline phosphatase, ATPase, alanyl amino-peptidase and leucine aminopeptidase were absent in the epidermal cells. SDH, cytochrome oxidase, MAO and a certain number of NAD-dependent dehydrogenases (LDH, ADH, MDH, alpha-GPDH, beta-OHBDH and GDH) showed a stronger reactivity in the basal cells and Malpighian layer. The NADP-dependent enzymes (G-6-PDH, 6-PGDH, cis-aconistase and ICDH) were more reactive in the upper Malpighian layer and stratum granulosum. The stratum corneum showed some acid phosphatase and nonspecific esterase reactivity. The collagenous fibers intertwined with a small number of very thin elastic ones and a larger amount of reticular fibers run almost parallel to the epidermal ridges in the papillary body. In the reticular dermis some fibers are disposed transversely to the epidermal ridges. Meissner corpuscles reactive to butyrylcholinesterase, acetylcholinesterase, nonspecific esterase and G-6-PA are disposed at regular intervals and frequently at each side of the epidermal ridges. Pacinian corpuscles were found only in the hypodermis. The eccrine sweat glands contain glycogen, UDPG-GT and phosphorylase in their secretory, ductal and myoepithelial cells. The secretory part shows a uniform reactivity for every dehydrogenase because it contains only one type of cells (clear cells). The intraepidermal segment of the ducts shows a stronger reactivity to nonspecific esterase and NADP-dependent dehydrogenases than the epithelial cells around it.
对4只成年雄性狨猴（2只普通狨猴和2只髭狨猴）的手掌和足底皮肤的结构及组织化学进行了研究。在这种皮肤中，存在发育良好的表皮嵴，每个表皮嵴附着有1或2条汗腺导管。表皮可见较厚的角质层，而透明层无法与其他层区分开来。颗粒层由1或3层含有透明角质颗粒的细胞组成。黑色素颗粒主要集中在表皮嵴的基底细胞中。在表皮细胞中发现了树突状黑素细胞和含有碱性磷酸酶的无黑素黑素细胞。糖原、尿苷二磷酸葡萄糖 - 糖基转移酶（UDPG - GT）和磷酸化酶主要存在于表皮嵴的中、下部马尔皮基层细胞中。表皮细胞中不存在碱性磷酸酶、三磷酸腺苷酶（ATPase）、丙氨酰氨基肽酶和亮氨酸氨基肽酶。琥珀酸脱氢酶（SDH）、细胞色素氧化酶、单胺氧化酶（MAO）和一些依赖烟酰胺腺嘌呤二核苷酸（NAD）的脱氢酶（乳酸脱氢酶（LDH）、乙醇脱氢酶（ADH）、苹果酸脱氢酶（MDH）、α - 甘油磷酸脱氢酶（α - GPDH）、β - 羟丁酸脱氢酶（β - OHBDH）和谷氨酸脱氢酶（GDH））在基底细胞和马尔皮基层表现出较强的反应性。依赖烟酰胺腺嘌呤二核苷酸磷酸（NADP）的酶（葡萄糖 - 6 - 磷酸脱氢酶（G - 6 - PDH）、6 - 磷酸葡萄糖酸脱氢酶（6 - PGDH）、顺乌头酸酶和异柠檬酸脱氢酶（ICDH））在上部马尔皮基层和颗粒层反应性更强。角质层表现出一些酸性磷酸酶和非特异性酯酶反应性。在乳头体中，胶原纤维与少量非常细的弹性纤维以及大量网状纤维交织在一起，几乎与表皮嵴平行排列。在网状真皮中，一些纤维与表皮嵴横向排列。对丁酰胆碱酯酶、乙酰胆碱酯酶、非特异性酯酶和葡萄糖 - 6 - 磷酸酶（G - 6 - PA）有反应的梅克尔小体以规则的间隔排列，并且经常位于表皮嵴的两侧。仅在皮下组织中发现了环层小体。外泌汗腺的分泌细胞、导管细胞和肌上皮细胞中含有糖原、UDPG - GT和磷酸化酶。分泌部对每种脱氢酶都表现出均匀的反应性，因为它只包含一种类型的细胞（透明细胞）。导管的表皮内段对非特异性酯酶和依赖NADP的脱氢酶的反应性比其周围的上皮细胞更强。
