Nakamura S, Takeda Y, Yoshida T, Ohtake S, Kobayashi K, Kanno M, Matano S, Matsuda T
Third Department of Internal Medicine, Kanazawa University School of Medicine.
Nihon Ketsueki Gakkai Zasshi. 1990 May;53(3):559-66.
Silver staining of nucleolar organizer regions (NOR) was applied to air-dried peripheral and bone marrow smears of normal subjects and leukemic patients. Specimens were fixed in buffered acetone formalin. Even in smears kept for 2 years at room temperature, the stainability of Ag-NOR was well preserved. By dipping Giemsa-stained smears in 5% trichloracetic acid and then placing them in methanol for 5 minutes, the stain was leached out. After the dye had been removed, the smears were clearly stained by a Ag-NOR staining technique. The mean number of Ag-NOR per nucleus of mature granulocytes and mononuclear cells in normal peripheral bloods was 0.59 and 1.43 respectively. The mean number of Ag-NOR per nucleus of peripheral and bone marrow leukemic cells from patients with acute leukemia and chronic myelocytic leukemia in blastic crisis was 2.32 and 2.66 respectively. On the other hand, the mean number of Ag-NOR per nucleus of peripheral leukemic cells from patients with chronic lymphocytic leukemia was 1.48. These results suggest that acute leukemia cells possess a more active proliferating potential. The Ag-NOR staining technique is very simple and might be useful for investigation of hematologic cells.
将核仁组织区（NOR）银染法应用于正常人和白血病患者的外周血及骨髓涂片空气干燥标本。标本用缓冲丙酮福尔马林固定。即使在室温下保存2年的涂片，银染核仁组织区（Ag-NOR）的染色性也保存良好。将吉姆萨染色的涂片浸入5%三氯乙酸中，然后置于甲醇中5分钟，可洗去染色。去除染料后，涂片用Ag-NOR染色技术清晰染色。正常外周血中成熟粒细胞和单核细胞的核平均Ag-NOR数分别为0.59和1.43。急性白血病和慢性粒细胞白血病急变期患者外周血及骨髓白血病细胞核平均Ag-NOR数分别为2.32和2.66。另一方面，慢性淋巴细胞白血病患者外周血白血病细胞核平均Ag-NOR数为1.48。这些结果提示急性白血病细胞具有更活跃的增殖潜能。Ag-NOR染色技术非常简单，可能有助于血液学细胞的研究。
