Mamaev N N, Mamaeva S E, Liburkina I L, Kozlova T V, Medvedeva N V
Tsitologiia. 1984 Jan;26(1):46-51.
The nucleolus organizer activity of bone marrow cell chromosomes has been studied by silver staining (Howell, Black, 1980) for 6 healthy donors, 24 patients with acute leukemia, and 17 patients with chronic myelocytic leukemia, including 6 cases of blast crisis. In respect to the nucleolus organizer silver staining pattern, bone marrow cells of donors and of patients with leukemias appeared to be more heterogeneous than PHA-stimulated lymphocytes of the same individuals. The average numbers of Ag-stained nucleolus organizers per metaphase in donors (5.2 +/- 0.22), patients with chronic phases (4.9 +/- 0.30), and patients with blast crisis of myelocytic leukemia (5.3 +/- 0.37), and in those with acute leukemia (5.2 +/- 0.46) were lower than those in PHA-stimulated lymphocytes of the same individuals; a great part of bone marrow cell mitoses (from 19 up to 46%) being Ag-negative in all the above groups. A conclusion is made that the heterogeneity in silver staining of the nucleolus organizers in bone marrow cells is due mostly to differences in their mitotic cell maturity degrees. Prospects of employment of silver staining of nucleolus organizers in cells for clinical purposes are discussed.
通过银染法（豪厄尔、布莱克，1980年）研究了6名健康供者、24名急性白血病患者和17名慢性粒细胞白血病患者（包括6例急变期患者）骨髓细胞染色体的核仁组织区活性。就核仁组织区银染模式而言，供者和白血病患者的骨髓细胞似乎比同一患者经PHA刺激的淋巴细胞更具异质性。供者中期每个细胞Ag染色核仁组织区的平均数（5.2±0.22）、慢性期患者（4.9±0.30）、慢性粒细胞白血病急变期患者（5.3±0.37）以及急性白血病患者（5.2±0.46）均低于同一患者经PHA刺激的淋巴细胞；上述所有组中很大一部分骨髓细胞有丝分裂（19%至46%）为Ag阴性。得出的结论是，骨髓细胞核仁组织区银染的异质性主要是由于其有丝分裂细胞成熟度的差异。讨论了细胞内核仁组织区银染用于临床的前景。
