基于氟化荧光素的具有光稳定性且具备荧光共振能量转移能力的改进型双砷-四半胱氨酸探针。
Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins.
作者信息
Spagnuolo Carla C, Vermeij Rudolf J, Jares-Erijman Elizabeth A
机构信息
Departamento de Química Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Ciudad Universitaria-Pabellón II, 1428, Buenos Aires, Argentina.
出版信息
J Am Chem Soc. 2006 Sep 20;128(37):12040-1. doi: 10.1021/ja063212q.
We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 A) than any previously obtained with biarsenical dyes.
我们已开发出双砷-四半胱氨酸标记物FlAsH的氟取代版本，与原始的荧光素衍生物相比，其重要特性有显著改善。在与四半胱氨酸靶标的复合物中，F2FlAsH的光稳定性比FlAsH提高了50倍，pH敏感性更低，吸光度和量子产率更高，并且F4FlAsH为双砷染料的调色板增添了一种新颜色。这两种探针还提供了一对新的荧光共振能量转移（FRET）对，其Förster半径（Ro值）为54 Å，比之前用双砷染料获得的任何值都大。
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