用于对四半胱氨酸标记蛋白进行荧光标记的膜渗透性双砷试剂FlAsH-EDT2和ReAsH-EDT2的制备。
Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.
作者信息
Adams Stephen R, Tsien Roger Y
机构信息
Department of Pharmacology, University of California, San Diego, La Jolla, California 92093-0647, USA.
出版信息
Nat Protoc. 2008;3(9):1527-34. doi: 10.1038/nprot.2008.144.
Abstract
The membrane-permeant fluorogenic biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin, respectively. Handling of toxic reagents such as arsenic trichloride is minimized so the synthesis can be carried out in a typical chemistry laboratory, usually taking about 2-3 d. A wide range of other biarsenical reagents and intermediates that also bind to tetracysteine-tagged (CysCysProGlyCysCys) proteins can be prepared similarly using this general procedure.
摘要
可渗透细胞膜的荧光双砷试剂FlAsH-EDT(2)和ReAsH-EDT(2)，分别通过简单的两步法，由廉价的前体染料荧光素和试卤灵高产率制备。三氯化砷等有毒试剂的处理降至最低限度，因此合成可在典型的化学实验室中进行，通常耗时约2-3天。使用这一通用方法，同样可以制备一系列能与四半胱氨酸标签（CysCysProGlyCysCys）蛋白结合的其他双砷试剂及中间体。
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