Artzy Schnirman Arbel, Zahavi Efrat, Yeger Hadas, Rosenfeld Ronit, Benhar Itai, Reiter Yoram, Sivan Uri
Department of Biology, and The Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Nano Lett. 2006 Sep;6(9):1870-4. doi: 10.1021/nl0607636.
Seamless integration of biomolecules with manmade materials will most likely rely on molecular recognition and specific binding. In the following we show that combinatorial antibody libraries, based on the vast repertoire of the human immune system, can be harnessed to generate such binders. As a demonstration, we isolate antibody fragments that discriminate and bind selectively GaAs (111A) facets as opposed to GaAs (100). The isolated antibodies are utilized for exclusive localization of a fluorescent dye on (111A) surfaces in a structure comprising a mixture of (100) and (111A) surfaces. The potential importance of structure rigidity to facet recognition is suggested vis-a-vis published experiments with short and longer peptides.
生物分子与人工合成材料的无缝整合很可能依赖于分子识别和特异性结合。在接下来的内容中，我们展示了基于人类免疫系统丰富库的组合抗体文库可用于生成此类结合物。作为一个例证，我们分离出了能够区分并选择性结合砷化镓（111A）面而非砷化镓（100）面的抗体片段。所分离出的抗体被用于在包含（100）面和（111A）面混合物的结构中，使荧光染料在（111A）表面进行特异性定位。相对于已发表的关于短肽和长肽的实验，结构刚性对晶面识别的潜在重要性也得到了体现。
