Kim Hyun-Jin, Chen Feng, Wang Xi, Choi Ju-Hee
Department of Food Science and Human Nutrition, Clemson University, Clemson, South Carolina 29634, USA.
J Agric Food Chem. 2006 Sep 20;54(19):7263-9. doi: 10.1021/jf060568c.
The effect of spraying exogenous plant hormone methyl jasmonate (MeJA) upon radish sprout (Raphanus sativus L.) was investigated in aspects of total phenolic content (TPC), isothiocyanate content, antioxidant activity of the radish extract, and enzymatic activities of phenylalanine ammonia lyase (PAL) and myrosinase. The MeJA treatment significantly increased the TPC that resulted in the increased DPPH* (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capacity. In addition, the PAL activity also increased by 60% at 24 h after MeJA treatment. However, the same treatment decreased the amount of 4-methylthio-3-butenylisothiocyanate (MTBITC), a major isothiocyanate in radish sprout and the activity of myrosinase, an enzyme related to produce isothiocyanates.
研究了喷施外源植物激素茉莉酸甲酯（MeJA）对萝卜芽（Raphanus sativus L.）总酚含量（TPC）、异硫氰酸酯含量、萝卜提取物抗氧化活性以及苯丙氨酸解氨酶（PAL）和黑芥子酶酶活性的影响。MeJA处理显著提高了TPC，导致二苯基苦味酰基自由基（DPPH*）清除能力增强。此外，MeJA处理24小时后PAL活性也提高了60%。然而，相同处理降低了萝卜芽中主要异硫氰酸酯4-甲硫基-3-丁烯基异硫氰酸酯（MTBITC）的含量以及与异硫氰酸酯产生相关的黑芥子酶的活性。
