Payet Bertrand, Shum Cheong Sing Alain, Smadja Jacqueline
Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, Faculté des Sciences et Technologies, Université de la Réunion, 15 avenue René Cassin, 97715 Saint Denis Messag. Cedex 9, La Réunion, France.
J Agric Food Chem. 2006 Sep 20;54(19):7270-6. doi: 10.1021/jf060808o.
Polyphenol content and free radical scavenging capacity of seven kinds of sugar manufacturing products (A sugars, clear juices, syrups, massecuite, and A, B, and C molasses) were studied. Seventy-two samples were collected at different stages of the process during two sugar harvests from a local sugar factory (Bois-Rouge, La Réunion). The total phenolic content of sugar products was determined according to the Folin-Ciocalteu assay. Polyphenols of sugar products were extracted with ethyl acetate and quantified by LC-UV-ESI-MS during all of the process. ABTS and DPPH assays were applied to aqueous solution of sugar products, which exhibited interesting free radical scavenging activity. Comparatively, ethyl acetate extracts exhibited higher antioxidant activity. Multivariate analyses (principal component analysis and canonical discriminant analysis) demonstrated a significant correlation between polyphenols and antioxidant activity. Moreover, it was observed that the sugar process results in an increase of the phenolic content and the free radical scavenging capacity of the different products. These products and especially molasses proved to be a rich source of natural antioxidants and may represent an interesting alternative to synthetic food antioxidants.
研究了七种制糖产品（A糖、清汁、糖浆、糖膏以及A、B和C糖蜜）的多酚含量和自由基清除能力。在两个制糖季期间，从当地一家制糖厂（留尼汪岛的布瓦鲁日）生产过程的不同阶段收集了72个样品。根据福林-酚试剂法测定糖产品的总酚含量。在整个过程中，用乙酸乙酯提取糖产品中的多酚，并通过液相色谱-紫外-电喷雾电离质谱法定量。将ABTS和DPPH试验应用于糖产品的水溶液，其表现出有趣的自由基清除活性。相比之下，乙酸乙酯提取物表现出更高的抗氧化活性。多变量分析（主成分分析和典型判别分析）表明多酚与抗氧化活性之间存在显著相关性。此外，观察到制糖过程会导致不同产品的酚含量和自由基清除能力增加。这些产品，尤其是糖蜜，被证明是天然抗氧化剂的丰富来源，可能是合成食品抗氧化剂的一个有趣替代品。
