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采用 DPPH、ABTS、FRAP、SOD 和 ORAC 法对 30 种具有工业应用价值的植物提取物的抗氧化性能和总酚含量进行比较研究。

Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays.

机构信息

Biolandes, Route de Belis, 40420 Le Sen, France.

出版信息

J Agric Food Chem. 2009 Mar 11;57(5):1768-74. doi: 10.1021/jf803011r.

Abstract

Aqueous extracts of 30 plants were investigated for their antioxidant properties using DPPH and ABTS radical scavenging capacity assay, oxygen radical absorbance capacity (ORAC) assay, superoxide dismutase (SOD) assay, and ferric reducing antioxidant potential (FRAP) assay. Total phenolic content was also determined by the Folin-Ciocalteu method. Antioxidant properties and total phenolic content differed significantly among selected plants. It was found that oak (Quercus robur), pine (Pinus maritima), and cinnamon (Cinnamomum zeylanicum) aqueous extracts possessed the highest antioxidant capacities in most of the methods used, and thus could be potential rich sources of natural antioxidants. These extracts presented the highest phenolic content (300-400 mg GAE/g). Mate (Ilex paraguariensis) and clove (Eugenia caryophyllus clovis) aqueous extracts also showed strong antioxidant properties and a high phenolic content (about 200 mg GAE/g). A significant relationship between antioxidant capacity and total phenolic content was found, indicating that phenolic compounds are the major contributors to the antioxidant properties of these plants.

摘要

研究了 30 种植物的水提物的抗氧化性能,使用 DPPH 和 ABTS 自由基清除能力测定法、氧自由基吸收能力(ORAC)测定法、超氧化物歧化酶(SOD)测定法和铁还原抗氧化能力(FRAP)测定法。还通过福林-希卡尔特法测定了总酚含量。所选植物的抗氧化性能和总酚含量存在显著差异。结果发现,栎(Quercus robur)、松(Pinus maritima)和肉桂(Cinnamomum zeylanicum)水提物在大多数使用的方法中具有最高的抗氧化能力,因此可能是天然抗氧化剂的潜在丰富来源。这些提取物的酚类含量最高(300-400mgGAE/g)。马黛茶(Ilex paraguariensis)和丁香(Eugenia caryophyllus clovis)水提取物也表现出很强的抗氧化性能和高的酚类含量(约 200mgGAE/g)。抗氧化能力与总酚含量之间存在显著的相关性,表明酚类化合物是这些植物抗氧化性能的主要贡献者。

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