Mancilla-Álvarez Eucario, Serrano-Fuentes María Karen, Fuentes-Torres María Angélica, Sánchez-Páez Ricardo, Bello-Bello Jericó Jabín
Postgraduate College-Campus Cordoba, Amatlan de los Reyes, Veracruz 94953, Mexico.
SECIHTI-Postgraduate College-Campus Cordoba, Amatlan de los Reyes, Veracruz 94953, Mexico.
Plants (Basel). 2025 Jun 1;14(11):1697. doi: 10.3390/plants14111697.
Chitosan nanoparticles (CsNPs) are biocompatible, biodegradable, and non-toxic natural polymers at low concentrations with diverse applications in in vitro plant tissue culture. This study aims to evaluate the effect of CsNPs during in vitro multiplication of sugarcane ( spp.) using temporary immersion bioreactors. CsNPs were evaluated at concentrations of 0, 25, 50, 100, and 200 mg L in Murashige and Skoog liquid culture medium. After four weeks of culture, response percentage, the number of shoots per explant, shoot length, number of leaves per explant, dry matter, chlorophyll content, -carotene content, lipid peroxidation, phenolic content, hydrogen peroxide content, and antioxidant capacity were evaluated. The results showed that the highest response percentages were obtained in the treatments with 0, 25, and 50 mg L CsNPs, whereas the lowest response percentages were obtained in the treatments with 100 and 200 mg L CsNPs. Concentrations of 25 and 50 mg L CsNPs promoted cell growth and differentiation, whereas 100 and 200 mg L CsNPs inhibited it. Chlorophyll content increased by 25 and 50 mg L-1 CsNPs, whereas -carotene content increased by 100 and 200 mg L CsNPs. Lipid peroxidation and antioxidant capacity increased with increasing CsNP concentrations. The phenolic content increased by 100 mg L CsNPs, whereas the hydrogen peroxide content decreased with increasing CsNP concentrations. In conclusion, CsNPs are an alternative for stimulating tissue growth and differentiation during the in vitro multiplication of sugarcane.
壳聚糖纳米颗粒（CsNPs）具有生物相容性、可生物降解性，且在低浓度下无毒，是天然聚合物，在体外植物组织培养中有多种应用。本研究旨在评估CsNPs在甘蔗（品种）使用临时浸没式生物反应器进行体外增殖过程中的作用。在Murashige和Skoog液体培养基中，对浓度为0、25、50、100和200 mg/L的CsNPs进行了评估。培养四周后，评估了响应率、每个外植体的芽数、芽长、每个外植体的叶数、干物质、叶绿素含量、β-胡萝卜素含量、脂质过氧化、酚类含量、过氧化氢含量和抗氧化能力。结果表明，在0、25和50 mg/L CsNPs处理中获得了最高的响应率，而在100和200 mg/L CsNPs处理中获得了最低的响应率。25和50 mg/L CsNPs的浓度促进了细胞生长和分化，而100和200 mg/L CsNPs则抑制了细胞生长和分化。25和50 mg/L-1 CsNPs使叶绿素含量增加，而100和200 mg/L CsNPs使β-胡萝卜素含量增加。脂质过氧化和抗氧化能力随CsNP浓度的增加而增加。100 mg/L CsNPs使酚类含量增加，而过氧化氢含量随CsNP浓度的增加而降低。总之，CsNPs是甘蔗体外增殖过程中刺激组织生长和分化的一种替代物。
