Niderkorn V, Boudra H, Morgavi D P
French Institute for Agricultural Research (INRA), Clermont-Fd-Theix Research Centre, Herbivore Research Unit, Saint Genès-Champanelle, France.
J Appl Microbiol. 2006 Oct;101(4):849-56. doi: 10.1111/j.1365-2672.2006.02958.x.
Fusarium toxins can occur in conserved forages impairing farm animal performances and health. On-farm biological decontamination methods could be an alternative to traditional physico-chemical methods. In this work, the ability to remove Fusarium toxins by fermentative bacteria was evaluated in vitro.
Twenty-nine strains of lactic (LAB) and propionic acid bacteria (PAB) were tested for their ability to remove deoxynivalenol (DON) and fumonisins B1 and B2 (FB1, FB2) from an acid, pH 4, medium. Mycotoxin removal was widespread for LAB, but differences among strains were large. Removal was up to 55% for DON, 82% for FB1 and 100% for FB2. Selected strains were also capable of removing up to 88% zearalenone. The PAB strains were less efficient than the LAB. Binding, not biodegradation appeared to be the mode of action, as no toxin derivatives were observed and removal was not impaired in nonviable bacteria. Binding was not affected by pH, except for fumonisins that decreased to nearly 0% at neutral pH.
Selected fermentative bacteria are able to bind main Fusarium mycotoxins.
The binding ability of selected strains could be used to decrease the bioavailability of toxins in contaminated silages.
镰刀菌毒素可出现在保存的饲料中，损害农场动物的生产性能和健康。农场生物去污方法可能是传统物理化学方法的一种替代方法。在这项研究中，体外评估了发酵细菌去除镰刀菌毒素的能力。
测试了29株乳酸菌（LAB）和丙酸菌（PAB）从pH值为4的酸性培养基中去除脱氧雪腐镰刀菌烯醇（DON）、伏马菌素B1和B2（FB1、FB2）的能力。乳酸菌对霉菌毒素的去除很普遍，但菌株间差异很大。DON的去除率高达55%，FB1为82%，FB2为100%。所选菌株还能够去除高达88%的玉米赤霉烯酮。丙酸菌菌株的效率低于乳酸菌。作用方式似乎是结合而非生物降解，因为未观察到毒素衍生物，且在非活细菌中去除不受影响。结合不受pH值影响，但伏马菌素在中性pH值下降至近0%。
所选发酵细菌能够结合主要的镰刀菌霉菌毒素。
所选菌株的结合能力可用于降低受污染青贮饲料中毒素的生物有效性。
