在无血清培养中，用纯化的重组粒细胞集落刺激因子刺激后的人粒细胞集落形成。
Human granulocyte colony formation in serum-free cultures stimulated with purified recombinant granulocyte colony-stimulating factor.
作者信息
Ieki R, Kudoh S, Kimura H, Ozawa K, Asano S, Takaku F
机构信息
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
出版信息
Exp Hematol. 1990 Sep;18(8):883-7.
Granulocyte colony formation by human bone marrow cells in serum-free cultures was studied using purified recombinant granulocyte colony-stimulating factor (rG-CSF). The cloning efficiency of the serum-free cultures was about 80% that of the serum-containing cultures. In addition to purified G-CSF, four ingredients were found to be essential to granulocyte colony formation: bovine serum albumin (BSA), iron-saturated human transferrin, cholesterol, and L-alpha-phosphatidylcholine. Their optimal concentrations were also investigated. Insulin was not indispensable for granulocyte colony formation, but its addition did increase the number of granulocyte colonies. Hydrocortisone was found to be inhibitory to granulocyte colony formation at high concentrations.
使用纯化的重组粒细胞集落刺激因子（rG-CSF）研究了人骨髓细胞在无血清培养物中的粒细胞集落形成。无血清培养物的克隆效率约为含血清培养物的80%。除了纯化的G-CSF外，还发现四种成分对粒细胞集落形成至关重要：牛血清白蛋白（BSA）、铁饱和的人转铁蛋白、胆固醇和L-α-磷脂酰胆碱。还研究了它们的最佳浓度。胰岛素对于粒细胞集落形成不是必需的，但添加它确实增加了粒细胞集落的数量。发现高浓度的氢化可的松对粒细胞集落形成有抑制作用。
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