针对肺炎克雷伯菌的鼠单克隆抗体可预防致死性内毒素血症及由荚膜型肺炎克雷伯菌引起的实验性感染。
Murine monoclonal antibodies to Klebsiella pneumoniae protect against lethal endotoxemia and experimental infection with capsulated K. pneumoniae.
作者信息
Mandine E, Salles M F, Zalisz R, Guenounou M, Smets P
机构信息
Centre de Recherches Immunologiques Roussel-Uclaf, Laboratoires Cassenne, Osny, France.
出版信息
Infect Immun. 1990 Sep;58(9):2828-33. doi: 10.1128/iai.58.9.2828-2833.1990.
To prepare monoclonal antibodies (MAbs) directed against the core-lipid A fractions of smooth lipopoly-saccharide (LPS) from Klebsiella pneumoniae O1:K2, we immunized BALB/c mice with the LPS-associated proteins plus LPS. This preparation exposed the core-lipid A moiety, which is normally hidden in the micellar structure of classical LPS preparations. Among 10 MAbs selected for their reactivity with LPS-associated proteins plus LPS from K. pneumoniae O1:K2, 6 (3A3, 3C2, 3C4, 7D2, 11C3, and 12B6) were directed against the core fraction and 2 (6C5 and 10A5) were directed against the lipid A fraction. Only one (2A4) recognized the O antigen, and one (6D5) had an undefined specificity. When injected before challenge with K. pneumoniae O1:K2 LPS in galactosamine-sensitized mice, five of the MAbs (3C4, 6D5, 7D2, 11C3, and 12B6) provided protection in this model of lethal endotoxemia. MAb 7D2 was also protective in an experimental infection with capsulated K. pneumoniae O1:K2.
为制备针对肺炎克雷伯菌O1:K2光滑型脂多糖（LPS）核心脂质A部分的单克隆抗体（MAb），我们用LPS相关蛋白加LPS免疫BALB/c小鼠。这种制剂暴露了核心脂质A部分，该部分通常隐藏在经典LPS制剂的胶束结构中。在因与肺炎克雷伯菌O1:K2的LPS相关蛋白加LPS反应性而挑选出的10种MAb中，6种（3A3、3C2、3C4、7D2、11C3和12B6）针对核心部分，2种（6C5和10A5）针对脂质A部分。只有一种（2A4）识别O抗原，一种（6D5）具有不确定的特异性。在用半乳糖胺致敏的小鼠中，在用肺炎克雷伯菌O1:K2 LPS攻击前注射时，其中5种MAb（3C4、6D5、7D2、11C3和12B6）在这种致死性内毒素血症模型中提供了保护。MAb 7D2在肺炎克雷伯菌O1:K2的实验性感染中也具有保护作用。
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