Koehler Martin Johannes, König Karsten, Elsner Peter, Bückle Rainer, Kaatz Martin
Department of Dermatology and Allergology, Freidrich Schiller University, Jena, Germany.
Opt Lett. 2006 Oct 1;31(19):2879-81. doi: 10.1364/ol.31.002879.
Changes of dermal collagen and elastin content are characteristic for skin aging as well as for pathological skin conditions. To evaluate these changes, we used in vivo multiphoton laser tomography to measure two-photon excited autofluorescence (AF) and second harmonic generation (SHG). We tested 18 patients of all ages and calculated the SHG-to-AF aging index of dermis (SAAID). We observed a negative relationship between the SAAID and age, which was accelerated for the female (n=7) subgroup. The current findings are the first in vivo demonstration of this relationship, and they show that specific characteristics of aged skin such as the ratio of extracellular matrix components collagen and elastin can be evaluated by in vivo AF and SHG measurements using near-IR femtosecond laser pulses.
真皮中胶原蛋白和弹性蛋白含量的变化是皮肤老化以及病理性皮肤状况的特征。为了评估这些变化，我们使用体内多光子激光断层扫描来测量双光子激发自体荧光（AF）和二次谐波产生（SHG）。我们测试了18名各年龄段的患者，并计算了真皮的SHG与AF老化指数（SAAID）。我们观察到SAAID与年龄之间呈负相关，在女性（n = 7）亚组中这种相关性加速。目前的研究结果首次在体内证明了这种关系，并且表明可以通过使用近红外飞秒激光脉冲进行体内AF和SHG测量来评估老化皮肤的特定特征，如细胞外基质成分胶原蛋白和弹性蛋白的比例。
