Koehler Martin Johannes, Hahn Stefanie, Preller Anja, Elsner Peter, Ziemer Mirjana, Bauer Andrea, König Karsten, Bückle Rainer, Fluhr Joachim W, Kaatz Martin
Department of Dermatology and Allergology, Friedrich Schiller University, Jena, Germany.
Exp Dermatol. 2008 Jun;17(6):519-23. doi: 10.1111/j.1600-0625.2007.00669.x. Epub 2008 Jan 15.
Morphological changes in the dermal collagen and elastin fibre network are characteristic for skin ageing and for pathological skin conditions of the dermis.
To characterize pathological and physiological conditions by multiphoton laser scanning tomography (MLT) in vivo, it is necessary to investigate and identify morphological alterations related to ageing.
In vivo MLT was used to image two-photon excited autofluorescence (AF) and second harmonics generation (SHG) in human dermis of 18 volunteers of different ages. Criteria for the evaluation of age-dependent morphological changes in MLT images were fibre tension and morphology, network pattern, clot formation and image homogeneity. These criteria were weighted and a score was calculated.
The resulting MLT-based Dermis Morphology Score is correlated with age (R2 = -0.90) and with the previously published SHG to AF Ageing Index of Dermis (R2 = 0.66). The two groups of young (age 21-38) and old (age 66-84) volunteers showed a significant difference in MLT score values (P < 0.001).
We could demonstrate an in vivo relationship between morphological characteristics of human dermis assessed by MLT and age. The present score allows the semi-quantitative evaluation of specific morphological changes of the dermal fibre network in ageing skin by in vivo AF and SHG imaging. This method will be useful for diagnostics of pathological conditions and their differentiation from ageing effects.
真皮中胶原蛋白和弹性纤维网络的形态变化是皮肤老化及真皮病理性皮肤状况的特征。
为了通过多光子激光扫描断层扫描（MLT）在体内表征病理和生理状况，有必要研究和识别与衰老相关的形态学改变。
使用体内MLT对18名不同年龄志愿者的人真皮中的双光子激发自发荧光（AF）和二次谐波产生（SHG）进行成像。评估MLT图像中年龄依赖性形态学变化的标准包括纤维张力和形态、网络模式、凝块形成和图像均匀性。对这些标准进行加权并计算得分。
基于MLT的真皮形态学评分与年龄相关（R2 = -0.90），并与先前发表的真皮SHG与AF衰老指数相关（R2 = 0.66）。两组年轻（21 - 38岁）和老年（66 - 84岁）志愿者的MLT评分值存在显著差异（P < 0.001）。
我们能够证明通过MLT评估的人真皮形态特征与年龄之间的体内关系。目前的评分允许通过体内AF和SHG成像对衰老皮肤中真皮纤维网络的特定形态变化进行半定量评估。该方法将有助于病理状况的诊断及其与衰老效应的区分。
