Gyurcsányi Robert E, Lindner Ernõ
Department of General and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, 1111 Budapest, Hungary.
Cytometry A. 2006 Aug 1;69(8):792-804. doi: 10.1002/cyto.a.20276.
High-resolution spectroscopic imaging of the cross section of ion-selective membranes during real-time electrochemical measurements is termed spectroelectrochemical microscopy (SpECM). SpECM is aimed for optimizing the experimental conditions in mass transport controlled ion-selective electrode (ISE) membranes for improved detection limit.
The SpECM measurements are performed in a thin layer electrochemical cell. The key element of the cell is a membrane strip spacer ring assembly which forms a two compartment electrochemical cell. The cell is placed onto the stage of a microscope and the membrane strip is positioned in the center of the field of view. A slice of the image is focused onto the entrance slit of the imaging spectrometer.
SpECM has been used for the determination of the diffusion coefficients of different membrane ingredients and for the quantitative assessment of the charged site concentrations in ISE membranes and membrane plasticizers. In addition, changes in the concentration profiles of the ionophore (free and complexed) and charged mobile sites inside the ISE membranes are documented upon the application of large external voltages.
This account demonstrates the power and advantages of SpECM, a multispectral imaging method for investigations of mass transport processes in ISE membranes during electrochemical measurements.
在实时电化学测量过程中，对离子选择性膜横截面进行高分辨率光谱成像被称为光谱电化学显微镜（SpECM）。SpECM旨在优化传质控制离子选择性电极（ISE）膜中的实验条件，以提高检测限。
SpECM测量在薄层电化学池中进行。该池的关键元件是一个膜条间隔环组件，它形成了一个双室电化学池。将该池放置在显微镜载物台上，膜条位于视野中心。图像的一部分聚焦在成像光谱仪的入射狭缝上。
SpECM已用于测定不同膜成分的扩散系数，以及对ISE膜和膜增塑剂中荷电位点浓度进行定量评估。此外，在施加较大外部电压时，记录了ISE膜内离子载体（游离和络合）和荷电移动位点浓度分布的变化。
本报告展示了SpECM的强大功能和优势，它是一种用于研究电化学测量过程中ISE膜传质过程的多光谱成像方法。
