Xiao Cheng, Zhao Lin-hua, Lu Cheng
Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006 Aug;26(8):689-93.
To explore the differential gene expression of peripheral CD4+ among rheumatoid arthritis (RA) patients of cold or heat syndrome type with or without rheumatoid factor (RF).
Differential gene expression of peripheral CD4+ lymphocytes purified from fasting venous blood of RA patients and healthy subjects was studied using gene chip technique.
There were 55 differential genes between RA patients with and without RF, mainly involving those related with immune response and signal transduction. In patients with RF, 71 differential genes, mainly related with functional metabolism and immune response, and in those without RF, 70 related with functional metabolism were found between patients of cold and heat syndrome type respectively, all of them were not repetitive with the above-mentioned 55, and only 2 were found repetitive between the 70 and the 71 differential genes, mainly involving functional metabolism.
The differential genes between RA patients with or without RF are different with those between patients of heat and cold syndrome type, suggesting the TCM syndrome classification has its own basis of gene expression profile.
探讨类风湿关节炎（RA）寒证或热证患者伴或不伴类风湿因子（RF）外周血CD4⁺的差异基因表达情况。
采用基因芯片技术研究从RA患者及健康对照者空腹静脉血中纯化的外周血CD4⁺淋巴细胞的差异基因表达。
伴RF与不伴RF的RA患者之间有55个差异基因，主要涉及免疫应答和信号转导相关基因。在伴RF的患者中，寒证与热证患者之间分别有71个差异基因，主要与功能代谢和免疫应答相关；在不伴RF的患者中，寒证与热证患者之间有70个与功能代谢相关的差异基因，这些差异基因均与上述55个差异基因无重复，且70个与71个差异基因之间仅发现2个重复基因，主要涉及功能代谢。
伴RF与不伴RF的RA患者之间的差异基因与寒证和热证患者之间的差异基因不同，提示中医证候分类有其自身的基因表达谱基础。
