Mallik Rangan, Hage David S
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
J Sep Sci. 2006 Aug;29(12):1686-704. doi: 10.1002/jssc.200600152.
The combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography (AMC). This review will discuss the basic principles behind AMC and examine the types of supports and ligands that have been employed in this method. Approaches for placing affinity ligands in monoliths will be considered, including methods based on covalent immobilization, biospecific adsorption, entrapment, and the formation of coordination complexes. Several reported applications will then be presented, such as the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, immobilized metal-ion affinity chromatography, dye-ligand affinity chromatography, and biomimetic chromatography. Other applications that will be discussed are chiral separations and studies of biological interactions based on AMC.
整体式载体与选择性亲和配体结合作为固定相的联合使用，最近催生了一种称为亲和整体柱色谱法（AMC）的新方法。本综述将讨论AMC背后的基本原理，并考察该方法中使用的载体和配体类型。将考虑在整体柱中放置亲和配体的方法，包括基于共价固定、生物特异性吸附、包埋以及配位络合物形成的方法。然后将介绍一些已报道的应用，例如AMC在生物亲和色谱、免疫亲和色谱、固定化金属离子亲和色谱、染料配体亲和色谱和仿生色谱中的应用。还将讨论的其他应用是基于AMC的手性分离和生物相互作用研究。
