Laboratory of Organic Chemistry, Natural Products Chemistry Group, Wageningen University, Wageningen, The Netherlands.
J Sep Sci. 2010 Feb;33(3):422-38. doi: 10.1002/jssc.200900635.
Affinity chromatography on monolithic supports is a powerful analytical chemical platform because it allows for fast analyses, small sample volumes, strong enrichment of trace biomarkers and applications in microchips. In this review, the recent research using monolithic materials in the field of bioaffinity chromatography (including immunochromatography) is summarized and discussed. After giving an introduction into affinity chromatography, information on different biomolecules (antibodies, enzymes, lectins, aptamers) that can act as ligands in bioaffinity chromatography is presented. Subsequently, the history of monoliths, their advantages, preparation and formats (disks, capillaries and microchips) as well as ligand immobilization techniques are mentioned. Finally, analytical and preparative applications of bioaffinity chromatography on monoliths are presented. During the last four years 37 papers appeared. Protein A and G are still most often used as ligands for the enrichment of immunoglobulins. Antibodies and lectins remain popular for the analysis of mainly smaller molecules and saccharides, respectively. The highly porous cryogels modified with ligands are applied for the sorting of different cells or bacteria. New is the application of aptamers and phages as ligands on monoliths. Convective interaction media (epoxy CIM disks) are currently the most used format in monolithic bioaffinity chromatography.
整体柱上的亲和色谱是一种强大的分析化学平台，因为它允许快速分析、小样品量、痕量生物标志物的强烈富集以及在微芯片中的应用。在这篇综述中，总结和讨论了近年来在生物亲和色谱（包括免疫色谱）领域中使用整体材料的研究。在介绍亲和色谱之后，介绍了可以作为生物亲和色谱配体的不同生物分子（抗体、酶、凝集素、适体）的信息。随后，提到了整体柱的历史、它们的优点、制备和形式（盘、毛细管和微芯片）以及配体固定化技术。最后，介绍了整体柱上生物亲和色谱的分析和制备应用。在过去的四年中，出现了 37 篇论文。蛋白 A 和 G 仍然是最常用于富集免疫球蛋白的配体。抗体和凝集素仍然是分析小分子和糖的主要配体。用配体修饰的高多孔性冷冻凝胶用于不同细胞或细菌的分选。新的是将适体和噬菌体作为配体应用于整体柱上。目前，在整体柱生物亲和色谱中，最常用的形式是环氧 CIM 盘。
