在X染色体失活时，使用HpaII聚合酶链反应分析法研究小鼠胚胎Pgk-1 CpG岛中的DNA甲基化。
Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.
作者信息
Singer-Sam J, Grant M, LeBon J M, Okuyama K, Chapman V, Monk M, Riggs A D
机构信息
Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010.
出版信息
Mol Cell Biol. 1990 Sep;10(9):4987-9. doi: 10.1128/mcb.10.9.4987-4989.1990.
Abstract
A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.
摘要
采用HpaII-PCR分析方法研究单个小鼠胚胎中的DNA甲基化情况。结果发现，X染色体连锁的Pgk-1基因的CpG岛中的HpaII位点H-7在卵母细胞和雄性胚胎中的甲基化程度小于或等于10%，但在6.5天的雌性胚胎中甲基化程度达到40%，大约是内细胞团X染色体失活的时间。
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