小家鼠生殖细胞中重复DNA序列的甲基化模式。
Methylation patterns of repetitive DNA sequences in germ cells of Mus musculus.
作者信息
Sanford J, Forrester L, Chapman V, Chandley A, Hastie N
出版信息
Nucleic Acids Res. 1984 Mar 26;12(6):2823-36. doi: 10.1093/nar/12.6.2823.
The major and the minor satellite sequences of Mus musculus were undermethylated in both sperm and oocyte DNAs relative to the amount of undermethylation observed in adult somatic tissue DNA. This hypomethylation was specific for satellite sequences in sperm DNA. Dispersed repetitive and low copy sequences show a high degree of methylation in sperm DNA; however, a dispersed repetitive sequence was undermethylated in oocyte DNA. This finding suggests a difference in the amount of total genomic DNA methylation between sperm and oocyte DNA. The methylation levels of the minor satellite sequences did not change during spermiogenesis, and were not associated with the onset of meiosis or a specific stage in sperm development.
相对于成年体细胞组织DNA中观察到的低甲基化程度，小家鼠的主要和次要卫星序列在精子和卵母细胞DNA中均处于低甲基化状态。这种低甲基化是精子DNA中卫星序列所特有的。分散的重复序列和低拷贝序列在精子DNA中呈现高度甲基化；然而，一个分散的重复序列在卵母细胞DNA中处于低甲基化状态。这一发现表明精子和卵母细胞DNA之间总基因组DNA甲基化量存在差异。次要卫星序列的甲基化水平在精子发生过程中没有变化，并且与减数分裂的开始或精子发育的特定阶段无关。
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