高效液相色谱法测定强效阿育吠陀药物“Chyavanprash”中的酚类物质含量

High-performance liquid chromatographic method for the quantification of phenolics in 'Chyavanprash' a potent Ayurvedic drug.

作者信息

Govindarajan R, Singh D P, Rawat A K S

机构信息

Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow 226 001, India.

出版信息

J Pharm Biomed Anal. 2007 Jan 17;43(2):527-32. doi: 10.1016/j.jpba.2006.08.005. Epub 2006 Sep 12.

DOI:10.1016/j.jpba.2006.08.005

PMID:16971083

Abstract

Quantification of bioactive principles through modern analytical tools is essential for establishing the authenticity, credibility, prescription and usage of Ayurvedic medicines/herbal formulations. 'Chyavanprash' is one of the oldest and most popular Ayurvedic preparations, used widely as a health promotive and disease preventive 'Rasayana' drug in India and elsewhere. The rejuvenating and tonic properties of 'Chavanprash' are considered mainly due to their antioxidant principles, which in turn is due to the presence of phenolic compounds. A simple high-performance liquid chromatography (HPLC) method for the separation and quantitative determination of the major antioxidant compounds from 'Chyavanprash' has been developed. The use of Waters Symmetry column and an acidic mobile phase enabled the efficient separation of phenolic compounds (catechin, quercetin-3-O-rutinoside, syringic acid and gallic acid) within a 35 min analysis. Validation of the method was done with a view to demonstrate its selectivity, linearity, precision, accuracy and robustness. In addition optimization of the complete extraction of phenolic compounds were also studied.

摘要

通过现代分析工具对生物活性成分进行定量，对于确定阿育吠陀药物/草药配方的真实性、可信度、处方和用法至关重要。“Chyavanprash”是最古老且最受欢迎的阿育吠陀制剂之一，在印度及其他地区广泛用作促进健康和预防疾病的“Rasayana”药物。“Chavanprash”的 rejuvenating 和滋补特性主要被认为归因于其抗氧化成分，而这又归因于酚类化合物的存在。已开发出一种简单的高效液相色谱（HPLC）方法，用于从“Chyavanprash”中分离和定量测定主要抗氧化化合物。使用 Waters Symmetry 柱和酸性流动相能够在 35 分钟的分析时间内有效分离酚类化合物（儿茶素、槲皮素 -3-O-芸香糖苷、丁香酸和没食子酸）。对该方法进行了验证，以证明其选择性、线性、精密度、准确度和稳健性。此外，还研究了酚类化合物完全提取的优化。